UPLC-MS-based plasma metabolomics for identifying energy metabolism biomarkers of maintenance in growing pigs

The purpose of this study is to explore the potential plasma metabolism biomarkers reflecting the maintenance status of growing pigs. The repeated measurement design was used in this experiment, and six barrows (28.6 +/- 0.5 kg BW) were selected and kept in metabolism crates. The feeding level in growing pigs close to ad libitum was 2400 kJ ME/kg BW0.6 center dot day(-1) during Day 1 to Day 7, while a feeding level of 782 kJ ME/kg BW0.6 center dot day(-1) was provided as energy requirement for maintenance during Day 8 to Day 14. Plasma samples of each pig were collected from the anterior vena cava on the morning of Day 8 and Day 15. The metabolites of plasma were determined by high-resolution mass spectrometry using a metabolomics approach. Results showed that metabolomics analysis between ad libitum-fed state and maintained status revealed differences in 16 compounds. Identified compounds were enriched in metabolic pathways related to linoleic acid metabolism, tryptophan metabolism, and alanine, aspartate and glutamate metabolism. In conclusion, linoleic acid metabolism, tryptophan metabolism, alanine, aspartate and glutamic acid metabolism pathways played a major regulatory role in the maintenance status of growing pigs. The potential metabolism biomarkers of maintenance in growing pigs were linoleic acid, glutamine and tyrosine.

Automated summary

The purpose of this study is to explore potential plasma metabolism biomarkers reflecting the maintenance status of growing pigs. The results showed that linoleic acid metabolism, tryptophan metabolism, and alanine, aspartate, and glutamate metabolism pathways play a significant regulatory role in the maintenance status of growing pigs.