An Expeditious Spectrophotometric Estimation of Memantine Hydrochloride by Facile Derivatization Using N,N-Dimethyl Aniline

The present work deals with the development and validation of an UV-Vis spectrophotometric method for the quantitative determination of Memantine hydrochloride. To execute this task, Memantine (MMT), being an UV inactive molecule, was first derivatized by conducting conventional diazotization reaction and further coupling with N,N-dimethyl aniline (DMA) at 2 to 5 degrees C. The newly formed MMT-DMA complex showed characteristic absorbance maximum at 354 nm. The spectroscopic estimation was achieved by scanning the spectrum between 200 and 800 nm on Shimadzu UV-1900 spectrophotometer. The developed method was validated further with respect to linearity, accuracy, precision, LOD, LOQ and robustness. The method showed expediency over the previously reported methods including instantaneous reaction, facile synthesis and high sensitivity. Moreover, the method has proven to be successful when applied for quantitative determination of marketed pharmaceutical formulations.

Automated summary

This study developed and validated a UV-Vis spectrophotometric method for the quantitative determination of Memantine hydrochloride. The UV inactive molecule Memantine (MMT) was first derivatized through a conventional diazotization reaction and coupled with N,N-dimethyl aniline (DMA) to form the MMT-DMA complex, which exhibited a characteristic absorbance maximum at 354 nm. The method showed convenience over previous methods with instantaneous reaction, facile synthesis, and high sensitivity. It was successfully applied for the quantitative determination of marketed pharmaceutical formulations.