4.7 Article

Biochemical Characterization of a GH46 Chitosanase Provides Insights into the Novel Digestion Specificity

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

Biochemical characterization and cleavage pattern analysis of a novel chitosanase with cellulase activity

Haipeng Su et al.

Summary: In this study, a novel GH5 chitosanase OUC-Csngly with dual chitosanase and cellulase activities was cloned and characterized. Molecular docking analysis revealed that the C-2 group of sugar units influenced the binding of the enzyme to oligosaccharides, resulting in different cleavage patterns. Additionally, OUC-Csngly exhibited distinctive cleavage patterns towards two different types of oligosaccharides.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022)

Article Agriculture, Multidisciplinary

New Insights into Bifunctional Chitosanases with Hydrolysis Activity toward Chito- and Cello-Substrates

Haipeng Su et al.

Summary: In this study, we investigated the bifunctional activity of GH46 chitosanases based on cleavage specificity classification. We found that CsnMHK1 from Bacillus circulans MH-K1, previously thought to only hydrolyze chitosan, can also hydrolyze cello-substrates. Comparison with GH8 chitosanase provided new insights into substrate specificities and challenged the current understanding of chitosanase classification. These findings contribute to our understanding of chitosanase recognition and hydrolysis, which is valuable for future studies.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

Article Agriculture, Multidisciplinary

Characterization of a New Xylanase Found in the Rumen Metagenome and Its Effects on the Hydrolysis of Wheat

Die Hu et al.

Summary: A novel xylanase RuXyn from beef cattle rumen metagenome has the potential to degrade wheat xylan and produce xylooligosaccharides, which could improve the application of wheat in poultry production.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

Article Biotechnology & Applied Microbiology

Constitutive chitosanase from Bacillus thuringiensis B-387 and its potential for preparation of antimicrobial chitooligomers

Gleb E. Aktuganov et al.

Summary: The article demonstrates the high constitutive production of extracellular chitosanase by the entomopathogenic strain B. thuringiensis var. dendrolimus B-387. The chitosanase was purified and characterized biochemically, and it showed the ability to hydrolyze soluble chitosan and generate low-molecular weight chitosan with bioactive properties. The findings highlight the potential of chitosanase B-387 in the production of bioactive chitooligosaccharides.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2022)

Article Agriculture, Multidisciplinary

New Class of Chitosanase from Bacillus amyloliquefaciens for the Generation of Chitooligosaccharides

Bhoopal Bhuvanachandra et al.

Summary: Chitooligosaccharides (COS), derived from chitin or chitosan, have diverse applications. A chitosanase from Bacillus amyloliquefaciens (BamCsn) was characterized, with the crucial role of a tryptophan residue, W204, identified for its activity. BamCsn exhibited unique properties such as transglycosylation and the ability to cleave chitin oligosaccharides, showing potential for COS generation.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

Article Biotechnology & Applied Microbiology

Biochemical characterization of a novel bifunctional chitosanase from Paenibacillus barengoltzii for chitooligosaccharide production

Zhenqiang Jiang et al.

Summary: A novel chitosanase gene, PbCsn8, was cloned and expressed in Bacillus subtilis with the highest activity at 70 degrees Celsius and pH 5.5. It showed specificity towards chitosan and glucan, making it a potential candidate for industrial applications. High yield and remarkable hydrolysis properties suggest PbCsn8 could be valuable in industrial settings.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2021)

Article Biophysics

Structure-based rational design of chitosanase CsnMY002 for high yields of chitobiose

Yubin Li et al.

Summary: This research successfully prepared pure chitosan oligosaccharides using chitosanase from Bacillus subtilis MY002, providing a foundation for its future biomedical applications.

COLLOIDS AND SURFACES B-BIOINTERFACES (2021)

Article Chemistry, Applied

Engineering a carbohydrate binding module to enhance chitinase catalytic efficiency on insoluble chitinous substrate

Haipeng Su et al.

Summary: This study successfully modified the ChBDs of chitinase SaChiA4 to improve enzymatic activity, leading to advancements in industrial-scale hydrolysis and utilization of insoluble chitin.

FOOD CHEMISTRY (2021)

Article Chemistry, Multidisciplinary

The Pattern of Acetylation Defines the Priming Activity of Chitosan Tetramers

Sven Basa et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Biochemistry & Molecular Biology

High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation

Sa Luo et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Article Biotechnology & Applied Microbiology

Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data

Yanshuo Han et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

Review Food Science & Technology

Marine-polysaccharide degrading enzymes: Status and prospects

Huihui Sun et al.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2020)

Article Biochemistry & Molecular Biology

Structure-function analysis of Gynuella sunshinyii chitosanase uncovers the mechanism of substrate binding in GH family 46 members

Yani Wang et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Review Biochemistry & Molecular Biology

Chitosan oligosaccharide (COS): An overview

Muhammad Naveed et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Article Biotechnology & Applied Microbiology

Tunnel engineering to accelerate product release for better biomass-degrading abilities in lignocellulolytic enzymes

Zhenghui Lu et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Review Chemistry, Applied

Chitooligosaccharides and their biological activities: A comprehensive review

Fakhra Liaqat et al.

CARBOHYDRATE POLYMERS (2018)

Article Food Science & Technology

Biochemical properties of a novel chitosanase from Bacillus amyloliquefaciens and its use in membrane reactor

Zhen Qin et al.

LWT-FOOD SCIENCE AND TECHNOLOGY (2018)

Article Biochemistry & Molecular Biology

Cellobiohydrolases Produce Different Oligosaccharides from Chitosan

Gregor Tegl et al.

BIOMACROMOLECULES (2016)

Article Biochemistry & Molecular Biology

Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01

Qianqian Lyu et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2015)

Review Chemistry, Medicinal

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes

Pascal Viens et al.

MARINE DRUGS (2015)

Article Biotechnology & Applied Microbiology

Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp 1794 newly isolated from compost

Mina Zitouni et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2013)

Article Biochemistry & Molecular Biology

MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0

Koichiro Tamura et al.

MOLECULAR BIOLOGY AND EVOLUTION (2013)

Article Biotechnology & Applied Microbiology

Purification and characterization of a novel antifungal endo-type chitosanase from Anabaena fertilissima

Vishal Gupta et al.

ANNALS OF MICROBIOLOGY (2012)

Article Chemistry, Multidisciplinary

Gabedit-A Graphical User Interface for Computational Chemistry Softwares

Abdul-Rahman Allouche

JOURNAL OF COMPUTATIONAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Degradation of Chitosans with a Family 46 Chitosanase from Streptomyces coelicolor A3(2)

Ellinor B. Heggset et al.

BIOMACROMOLECULES (2010)

Article Biochemical Research Methods

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling

K Arnold et al.

BIOINFORMATICS (2006)

Article Biochemistry & Molecular Biology

Novel chitosanase from Streptomyces griseus HUT 6037 with transglycosylation activity

T Tanabe et al.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2003)