Turnover of Benzoxazinoids during the Aerobic Deterioration of Maize Silage (Zea mays)

While plant-specialized metabolites can affect mammal health, their fate during the aerobic deterioration of crop silage remains poorly understood. In this study, we investigated the metabolization of benzoxazinoids (BXs) in silages of two maize genotypes (W22 wild type and bx1 mutant line) during aerobic deterioration. In W22 plants, concentrations of the aglucone BXs DIMBOA and HMBOA in silage decreased over time upon air exposure, while concentrations of MBOA and BOA increased. Mutant plants had low levels of BXs, which did not significantly vary over time. Aerobic stability was BX-dependent, as pH and counts of yeasts and molds were higher in W22 compared to that in bx1 silage. The nutrient composition was not affected by BXs. These preliminary results may be used to estimate the amounts of BXs provided to farm animals via silage feeding. However, further research is warranted under different harvest and storage conditions.

Automated summary

This study investigates the metabolization of benzoxazinoids (BXs) in silages of two maize genotypes during aerobic deterioration. It finds that the concentration of BXs in silage decreases over time in the wild type maize, while the concentration increases in mutant maize. Aerobic stability is influenced by BXs, and the nutrient composition is not affected. Further research is needed under different conditions.