Research Advances Through Activity-Based Lipid Hydrolase Profiling

Activity-based proteomic profiling (ABPP) enables the functional study of enzymes by employing small molecule probes that bind covalently to the active site of an enzyme. Activity-based probes can penetrate cells and tissues and thereby allow enzymes to be targeted/labelled in their native state. Probes can be designed to target individual enzymes or whole enzyme groups, which makes ABPP a versatile protein profiling technique. In this review, we give an overview of research advances through ABPP in the context of lipid hydrolase research. We report of lipid hydrolases that were discovered and characterized through ABPP, and aim to give an overview of commonly used probes as well as inhibitors that were discovered and characterized by competitive ABPP. Lastly, this review aims to raise caveats and current limitations of this protein profiling technique.

Automated summary

Activity-based proteomic profiling (ABPP) is a versatile protein profiling technique that allows the functional study of enzymes by using small molecule probes that bind covalently to the active site of an enzyme. This technique enables the targeting and labeling of enzymes in their native state, even in cells and tissues. In the context of lipid hydrolase research, ABPP has been used to discover and characterize lipid hydrolases, as well as to identify commonly used probes and inhibitors through competitive ABPP. However, there are caveats and limitations that need to be considered when using this protein profiling technique.