Bridging biotechnology and nanomedicine to produce biogreen whey-nanovesicles for intestinal health promotion

New intestinal health-promoting biotechnological nanovesicles were manufactured by combining the main environmental pollutant generated from the cheese-making process, whey, with phospholipid, sodium hyaluronate and dextrin, thus overcoming environmental and medical challenges. An efficient, consolidated and ecofriendly preparation method was employed to manufacture the vesicles and the bioactive whey was obtained by mesophilic dark fermentation without external inoculum through a homolactic pathway, which was operated in such a way as to maximize the production of lactic acid. The biotechnological nutriosomes and hyalonutriosomes were relatively small (similar to 100 nm) and characterized by the net negative surface charge (>-30 mV). The addition of maltodextrin to the liposomes and especially to the hyalurosomes significantly stabilized the vesicles under acidic conditions, simulating the gastric environment, as their size and polydispersity index were significantly lower (p < 0.05) than those of the other formulations. The vesicles were effectively internalized by Caco-2 cells and protected them against oxidative stress. Nutriosomes promoted the proliferation of Streptococcus salivarius, a human commensal bacterium, to a better extent (p < 0.05) than liposomes and hyalurosomes, as a function of the concentration tested. These findings could open a new horizon in intestinal protection and health promotion by integrating biotechnology, nanomedicine, sustainability principles and bio-circular economy.

Automated summary

New intestinal health-promoting biotechnological nanovesicles were created by combining whey, a major environmental pollutant from cheese-making, with phospholipid, sodium hyaluronate, and dextrin. A consolidated and ecofriendly method was used to manufacture the vesicles, which were characterized by small size and negative surface charge. These vesicles were effectively internalized by Caco-2 cells and protected against oxidative stress. The findings suggest the potential of integrating biotechnology, nanomedicine, sustainability principles, and bio-circular economy for intestinal protection and health promotion.