Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/ijms24010218
Keywords
antimicrobial resistance; yeast mannan fraction; bacterial metabolism; antibiotic susceptibility; reactive oxygen species
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The Mannan-rich fraction (MRF) isolated from Saccharomyces cerevisiae has been found to be beneficial for animal intestinal health. In this study, it was observed that MRF affects the formation of reactive oxygen species (ROS) and antibiotic susceptibility in resistant Escherichia coli through modulation of bacterial metabolism. Furthermore, proteomics-based analysis revealed that MRF supplementation leads to significant changes in key metabolic pathways, including glycolysis-TCA cycle, thus enhancing bacterial susceptibility to antibiotics (p <= 0.05).
Mannan-rich fraction (MRF) isolated from Saccharomyces cerevisiae has been studied for its beneficial impact on animal intestinal health. Herein, we examined how MRF affected the formation of reactive oxygen species (ROS), impacting antibiotic susceptibility in resistant Escherichia coli through the modulation of bacterial metabolism. The role of MRF in effecting proteomic change was examined using a proteomics-based approach. The results showed that MRF, when combined with bactericidal antibiotic treatment, increased ROS production in resistant E. coli by 59.29 +/- 4.03% compared to the control (p <= 0.05). We further examined the effect of MRF alone and in combination with antibiotic treatment on E. coli growth and explored how MRF potentiates bacterial susceptibility to antibiotics via proteomic changes in key metabolic pathways. Herein we demonstrated that MRF supplementation in the growth media of ampicillin-resistant E. coli had a significant impact on the normal translational control of the central metabolic pathways, including those involved in the glycolysis-TCA cycle (p <= 0.05).
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