4.7 Article

Definition and Characterization of SOX11-Derived T Cell Epitopes towards Immunotherapy of Glioma

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Publisher

MDPI
DOI: 10.3390/ijms24031943

Keywords

SOX11; glioma; HLA-A*0201; tumor-associated antigen; immunotherapy

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The transcription factor SOX11 is overexpressed in glioblastoma (GBM), and in silico prediction identified a novel peptide FMACSPVAL as a potential target for T cell-based immunotherapy. This peptide exhibited high binding affinity to the HLA-A*0201 molecule and generated SOX11-specific CD8(+) T cells in vitro. Positive responses to SOX11 were observed in 9 out of 32 healthy donors.
The transcription factor SOX11 is a tumor-associated antigen with low expression in normal cells, but overexpression in glioblastoma (GBM). So far, conventional surgery, chemotherapy, and radiotherapy have not substantially improved the dismal prognosis of relapsed/refractory GBM patients. Immunotherapy is considered a promising strategy against GBM, but there is a fervent need for better immunotargets in GBM. To this end, we performed an in silico prediction study on SOX11, which primarily yielded ten promising HLA-A*0201-restricted peptides derived from SOX11. We defined a novel peptide FMACSPVAL, which had the highest score according to in silico prediction (6.02 nM by NetMHC-4.0) and showed an exquisite binding affinity to the HLA-A*0201 molecule in the peptide-binding assays. In the IFN-gamma ELISPOT assays, FMACSPVAL demonstrated a high efficiency for generating SOX11-specific CD8(+) T cells. Nine out of thirty-two healthy donors showed a positive response to SOX11, as assessed by the ELISPOT assays. Therefore, this novel antigen peptide epitope seems to be promising as a target for T cell-based immunotherapy in GBM. The adoptive transfer of in vitro elicited SOX11-specific CD8(+) T cells constitutes a potential approach for the treatment of GBM patients.

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