Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos

Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated Lnx2 in mouse preimplantation embryos. Expression analysis showed that Lnx2 is expressed in oocytes and preimplantation embryos. Lnx2-knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (Oct4), are increased in Lnx2 knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by Lnx2 knockdown. Collectively, our results show that Lnx2 is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that Lnx2 may be involved in transcriptionally regulating various genes implicated in early embryonic development.

Automated summary

In this study, the researchers found that the E3 ubiquitin ligase LNX2 plays an important role in early embryonic development by regulating Notch signaling through the degradation of NUMB protein. Knockdown of LNX2 resulted in the majority of embryos failing to develop into normal blastocysts and increased expression levels of genes involved in cell lineage specification. These findings suggest that LNX2 may be involved in transcriptionally regulating various genes implicated in early embryonic development and may act via lineage specification of the inner cell mass.