4.7 Article

Dual CXCR4/IL-10 Gene-Edited Human Amniotic Mesenchymal Stem Cells Exhibit Robust Therapeutic Properties in Chronic Wound Healing

Journal

Publisher

MDPI
DOI: 10.3390/ijms232315338

Keywords

cell therapy; CXCR4; IL-10; gene editing; mesenchymal stem cells; wound healing

Funding

  1. National Research Foundation (NRF) of Korea - Korean Government [NRF-2022R1F1A1064405]
  2. research fund of Catholic Kwandong University
  3. NRF of Korea - Korean government [NRF-2020R1C1C101316611, NRF-2022R1F1A1068778]
  4. Dong-A University
  5. Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Republic of Korea [HI18C0661]

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In this study, enhanced therapeutic potential of amniotic mesenchymal stem cells (AMM) was investigated through dual CXCR4/IL-10 gene knock-in insertion, leading to accelerated wound healing, high angiogenic effects, and anti-inflammatory properties.
Although stem cells have attracted attention as a novel therapeutic solution for tissue regeneration, their minimal efficacy remains controversial. In the present study, we aimed to investigate the enhanced therapeutic property of CXCR4/IL-10 dual angiogenic/anti-inflammatory gene knock-in amniotic mesenchymal stem cells (AMM) in a wound-healing model. Dual CXCR4 and IL-10 genes were inserted into the AMM genome using transcription-activator-like effector nuclease (TALEN). Matrigel tube formation and anti-inflammatory effects were assessed in vitro, and efficacy was tested in vivo in a diabetic wound-healing model. CXCR4/IL-10-expressing amniotic MSCs (AMM/CI) strongly expressed CXCR4 and IL-10 genes and robustly promoted tube formation and anti-inflammatory potential. AMM/CI transplantation resulted in accelerated wound healing, as well as high engraftment and re-epithelialization potential. Transplanted AMM/CI also exhibited high angiogenic and decreased pro-inflammatory gene expression in the wound tissue, indicating direct therapeutic effects on wound healing. Taken together, these data indicate that dual angiogenic/anti-inflammatory gene knock-in may be a novel approach to enhance the therapeutic effects of stem cells, and transplantation of AMM/CI can be an alternative therapeutic option in chronic wound healing.

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