Accumulation of Anthocyanins in Detached Leaves of Kalanchoe blossfeldiana: Relevance to the Effect of Methyl Jasmonate on This Process

Accumulation of anthocyanins in detached leaves and in excised stems of Kalanchoe blossfeldiana kept under natural light conditions in the presence or absence of methyl jasmonate (JA-Me) was investigated. When the abaxial surface of detached leaves was held lower than the adaxial surface (the normal or natural position) under natural light conditions, anthocyanins were not accumulated on the abaxial side of the leaves. In contrast, when the adaxial surface of detached leaves was held lower than the abaxial surface (inverted position), anthocyanins were highly accumulated on the abaxial side of the leaves. These phenomena were independent of the growth stage of K. blossfeldiana as well as photoperiod. Application of JA-Me in lanolin paste significantly inhibited anthocyanin accumulation induced on the abaxial side of detached leaves held in an inverted position in a dose-dependent manner. Anthocyanin accumulation in the excised stem in response to natural light was also significantly inhibited by JA-Me in lanolin paste. Possible mechanisms of anthocyanin accumulation on the abaxial side of detached K. blossfeldiana leaves held in an inverted position under natural light conditions and the inhibitory effect of JA-Me on this process are described. The accompanying changes in the content of primary metabolites and histological analyses were also described.

Automated summary

The accumulation of anthocyanins in detached leaves and excised stems of Kalanchoe blossfeldiana under natural light conditions with or without methyl jasmonate (JA-Me) was investigated. Anthocyanins were found to accumulate on the abaxial side of the leaves when the adaxial surface was held lower than the abaxial surface, regardless of the growth stage and photoperiod. The application of JA-Me in lanolin paste inhibited the accumulation of anthocyanins in both the detached leaves and excised stems. Possible mechanisms and accompanying changes in primary metabolites and histology were described.