Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/ijms232113483
Keywords
nanoparticle; phagocytosis; Galleria mellonella; infection; cell culture
Funding
- MCIN/AEI [RTI2018-098573-B-I00, PID2021-125801OB100]
- ERDF A way of making Europe
- AGAUR-Generalitat de Catalunya [2017SGR-1079]
- European Regional Development Fund (FEDER)
- Catalan Cystic Fibrosis Association
- Generalitat de Catalunya [2021FI_B00118]
- CERCA program
- Obra Social La Caixa
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Galleria mellonella is an advantageous animal model for infection research, as it is easy and inexpensive to maintain and rear. Its use is ethically acceptable and it has a conveniently manipulable size. Additionally, its immune system shares similarities with mammalian immune systems. Hemocytes, the immune cells, play a crucial role in encapsulating and eliminating pathogens and foreign particles. However, culturing G. mellonella hemocytes in vitro presents challenges.
Galleria mellonella is an alternative animal model of infection. The use of this species presents a wide range of advantages, as its maintenance and rearing are both easy and inexpensive. Moreover, its use is considered to be more ethically acceptable than other models, it is conveniently sized for manipulation, and its immune system has multiple similarities with mammalian immune systems. Hemocytes are immune cells that help encapsulate and eliminate pathogens and foreign particles. All of these reasons make this insect a promising animal model. However, cultivating G. mellonella hemocytes in vitro is not straightforward and it has many difficult challenges. Here, we present a methodologically optimized protocol to establish and maintain a G. mellonella hemocyte primary culture. These improvements open the door to easily and quickly study the toxicity of nanoparticles and the interactions of particles and materials in an in vitro environment.
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