Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/ijms232213785
Keywords
electrochemical biosensor; antibiotics; tetracycline; cow milk samples; aptamer; ferrocene
Funding
- National Science Centre, Poland [2020/37/B/NZ9/03423]
- Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn
- EIT Food RIS Fellowships Action Line 2021
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In this paper, an electrochemical platform based on ssDNA aptamer for the detection of tetracycline in food products has been proposed. The developed biosensor shows simplicity, low detection limit, and high reliability, and has been successfully applied to the detection of tetracycline in cow milk.
Controlling food safety and preventing the growing spread of antibiotics into food products have been challenging problems for the protection of human health. Hence, the development of easy-to-use, fast, and sensitive analytical methods for the detection of antibiotics in food products has become one of the priorities in the food industry. In this paper, an electrochemical platform based on the ssDNA aptamer for the selective detection of tetracycline has been proposed. The aptasensor is based on a thiolated aptamer, labelled with ferrocene, which has been covalently co-immobilized onto a gold electrode surface with 6-mercaptohexan-1-ol. The changes in the redox activity of ferrocene observed on the aptamer-antibiotics interactions have been the basis of analytical signal generation registered by square-wave voltammetry. Furthermore, the detection of tetracycline-spiked cow milk samples has been successfully demonstrated. The limits of detection (LODs) have been obtained of 0.16 nM and 0.20 nM in the buffer and spiked cow milk, respectively, which exceed the maximum residue level (225 nM) more than 1000 times. The proposed aptasensor offers high selectivity for tetracycline against other structurally related tetracycline derivatives. The developed biosensor characterized by simplicity, a low detection limit, and high reliability shows practical potential for the detection of tetracycline in animal-origin milk.
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