Qinggan Huoxue Recipe attenuates Alcoholic Liver Disease by suppressing PI3K/AKT signaling pathway based on network pharmacology

Qinggan Huoxue Recipe (QGHXR) is originated from Xiao Chaihu Decotion. Many experimental studies have confirmed that QGHXR can significantly alleviate the symptoms of alcoholic liver disease (ALD), but the detailed mechanism is still unclear. Using traditional Chinese medicine network pharmacology analysis system database and animal experiments, we found that 180 potentially chemical compositions and 618 potential targets were screened from the prescription, which shared 133 signal pathways with ALD. Through animal experiments, it was found that QGHXR could reduce the liver total cholesterol (TC), serum TC, alanine aminotransferase, aspartate aminotransferase of ALD mice, reduce the lipid droplets and inflammatory injury of liver tissue. Meanwhile, it can also increase PTEN, decrease PI3K and AKT mRNA levels. In this study, we obtained the targets and pathways of QGHXR in the treatment of ALD, and preliminatively verified that QGHXR may improve ALD through PTEN/PI3K/AKT signaling pathway.

Automated summary

Qinggan Huoxue Recipe (QGHXR) is a traditional Chinese medicine originated from Xiao Chaihu Decotion, which has been shown to alleviate the symptoms of alcoholic liver disease (ALD). This study used Chinese medicine network pharmacology analysis and animal experiments to identify potential chemical compositions, targets, and pathways of QGHXR in treating ALD. The results suggest that QGHXR may improve ALD through the PTEN/PI3K/AKT signaling pathway by reducing liver cholesterol levels and inflammatory injury, while also affecting gene expression levels of PTEN, PI3K, and AKT.