4.7 Article

Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin

Related references

Note: Only part of the references are listed.
Review Biotechnology & Applied Microbiology

Improvement of the enzymatic detoxification activity towards mycotoxins through structure-based engineering

Yanxia Wang et al.

Summary: The contamination of food and feed by mycotoxins poses a serious threat to global food safety and public health. Enzymes have emerged as a promising approach for biological detoxification, but their low efficiency and stability limit their application. This review summarizes recent trends in structure-based engineering to improve the efficiency, selectivity, and stability of enzymes in detoxifying mycotoxins, providing insights into obtaining innovative and effective biocatalysts for mycotoxin degradation.

BIOTECHNOLOGY ADVANCES (2022)

Article Food Science & Technology

The characteristics of patulin degradation by probiotic yeast - Pichia guilliermondii S15-8

Ying Luo et al.

Summary: Probiotic yeast strain Pichia guilliermondii S15-8 showed high ability to degrade patulin in apple-derived products, with optimal degradation conditions established at 30 degrees C, 5 mL of juice simulated solution, and 24 hours of incubation, degrading over 90% of patulin. The yeast also exhibited strong tolerance to high levels of patulin stress, making it a potential candidate for detoxification of apple-derived products.

FOOD CONTROL (2022)

Article Biochemistry & Molecular Biology

A novel enzyme from Rhodotorula mucilaginosa Aldolase: Isolation, identification and degradation for patulin in apple juice

Na Li et al.

Summary: This study aimed to isolate and purify Aldolase from Rhodotorula mucilaginosa and investigate its ability to degrade patulin. The results showed that the purified Aldolase had good degradation efficiency and exhibited stability under certain conditions. This research provided the potential of Aldolase as a material for patulin degradation.

PROCESS BIOCHEMISTRY (2022)

Article Food Science & Technology

Characterization of Two Dehydrogenases from Gluconobacter oxydans Involved in the Transformation of Patulin to Ascladiol

Edicon T. S. Chan et al.

Summary: Patulin is a mycotoxin that contaminates apples and apple products, but can be transformed into E-ascladiol by extracts from Gluconobacter oxydans ATCC 621. The identification and characterization of enzymes involved in the transformation provide insights into their potential application for patulin detoxification.

TOXINS (2022)

Article Food Science & Technology

Patulin Detoxification by Recombinant Manganese Peroxidase from Moniliophthora roreri Expressed by Pichia pastoris

Shuai Wang et al.

Summary: Patulin, a mycotoxin commonly found in foods and beverages, can be efficiently degraded by a manganese peroxidase enzyme. The enzyme was able to rapidly degrade patulin and detoxify it, making it a potential candidate for enzymatic detoxification in food and beverages.

TOXINS (2022)

Review Biochemistry & Molecular Biology

Structure-based mechanisms: On the way to apply alcohol dehydrogenases/reductases to organic-aqueous systems

Yaohui Li et al.

Summary: This study summarizes the structures and behaviors of alcohol dehydrogenases/reductases in organic-aqueous systems, comparing the structures of enzymes with different tolerance towards organic solvents. By understanding the catalytic mechanisms of these enzymes in organic-aqueous systems, the efficient enantioselective syntheses mediated by alcohol dehydrogenases/reductases and further challenges are discussed through solvent engineering and enzyme immobilization in the last decade.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Article Agriculture, Multidisciplinary

Genomewide Transcriptome Responses of Arthrobacter simplex to Cortisone Acetate and its Mutants with Enhanced Δ1-Dehydrogenation Efficiency

Hongchen Jia et al.

Summary: This study revealed the molecular fundamentals and Delta(1)-dehydrogenation mechanism of Arthrobacter simplex through genome and transcriptome analysis, confirming the roles of key genes and constructing an optimal strain to increase prednisone acetate production and productivity.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

Article Chemistry, Physical

A Structural View on the Stereospecificity of Plant Borneol-Type Dehydrogenases

Andrea M. Chanique et al.

Summary: The study focused on utilizing dehydrogenases from Salvia species to investigate the enantiomer separation of borneol and isoborneol. A highly specific and stable dehydrogenase with remarkable activity for borneol and isoborneol was discovered, leading to the first three-dimensional structure of a specific borneol-type short-chain dehydrogenase. The identification of a hydrophobic pocket in the enzyme that plays a crucial role in stereo discrimination of bornane-type monoterpenoids was also achieved.

CHEMCATCHEM (2021)

Article Chemistry, Organic

Identification and Characterization of Enzymes Catalyzing Early Steps in Miharamycin and Amipurimycin Biosynthesis

Wen-He Zhang et al.

Summary: Biochemical elucidation of the early biosynthetic pathways of miharamycins and amipurimycin revealed the important roles of several enzymes, with some hypothetical proteins unexpectedly exhibiting dual functions. In addition, the functions of carbonyl reductase MihE and rare guanine O-methyltransferase MihF were also confirmed.

ORGANIC LETTERS (2021)

Article Engineering, Environmental

Insights into the highly efficient detoxification of the biotoxin patulin in water by discharge plasma oxidation

Mingming Xue et al.

Summary: The low-temperature discharge plasma approach is effective in detoxifying patulin in water and destroying the lactone and hemiacetal groups responsible for its toxicity. After treatment, acute oral toxicity in rats and developmental toxicity decreased, which was confirmed by cytotoxicity tests using yeast cells and HepG2 human hepatocellular carcinoma cells.

CHEMICAL ENGINEERING JOURNAL (2021)

Article Chemistry, Applied

Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice

Mengyang Xing et al.

Summary: The cloned SDR gene CgSDR can effectively transform patulin into E-ascladiol in vitro, showing significant degradation ability; addition of CgSDR can reduce 80% of patulin in apple juice without affecting its quality.

FOOD CHEMISTRY (2021)

Article Agriculture, Multidisciplinary

Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens

Mengyang Xing et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)

Article Materials Science, Multidisciplinary

Mussel-Inspired Membrane Adsorber with Thiol Ligand for Patulin Removal: Adsorption and Regeneration Behaviors

Lanfang Liu et al.

MACROMOLECULAR MATERIALS AND ENGINEERING (2019)

Review Food Science & Technology

The characteristics, occurrence, and toxicological effects of patulin

Iman Saleh et al.

FOOD AND CHEMICAL TOXICOLOGY (2019)

Review Food Science & Technology

The mycotoxin patulin: An updated short review on occurrence, toxicity and analytical challenges

Arnau Vidal et al.

FOOD AND CHEMICAL TOXICOLOGY (2019)

Article Agriculture, Multidisciplinary

S-Adenosylmethionine-Dependent Methyltransferase Helps Pichia caribbica Degrade Patulin

Kaili Wang et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019)

Review Food Science & Technology

Removing and detoxifying methods of patulin: A review

Enjie Diao et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2018)

Review Food Science & Technology

Mitigation of Patulin in Fresh and Processed Foods and Beverages

J. David Ioi et al.

TOXINS (2017)

Review Engineering, Chemical

Minireview: A Comeback of Hg-Derivatives in Protein Crystallography with Cys-Modification

Hsiu-Chien Chan et al.

CHEMBIOENG REVIEWS (2015)

Article Biotechnology & Applied Microbiology

Biochemical and Structural Studies of NADH-Dependent FabG Used To Increase the Bacterial Production of Fatty Acids under Anaerobic Conditions

Pouya Javidpour et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2014)

Article Biotechnology & Applied Microbiology

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

G. Ianiri et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2013)

Article Food Science & Technology

Survey of patulin in apple juice concentrates in Shaanxi (China) and its dietary intake

Yaodong Guo et al.

FOOD CONTROL (2013)

Review Food Science & Technology

A Review on Ozone-Based Treatments for Fruit and Vegetables Preservation

Fatima A. Miller et al.

FOOD ENGINEERING REVIEWS (2013)

Article Biochemistry & Molecular Biology

Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans

Xu Liu et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2011)

Article Agriculture, Multidisciplinary

Conversion of the Mycotoxin Patulin to the Less Toxic Desoxypatulinic Acid by the Biocontrol Yeast Rhodosporidium kratochvilovae Strain LS11

Raffaello Castoria et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2011)

Review Food Science & Technology

Biosynthesis and Toxicological Effects of Patulin

Olivier Puel et al.

TOXINS (2010)

Article Biochemistry & Molecular Biology

The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative

Bengt Persson et al.

CHEMICO-BIOLOGICAL INTERACTIONS (2009)

Article Biochemical Research Methods

Coot:: model-building tools for molecular graphics

P Emsley et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2004)