Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 222, Issue -, Pages 1343-1352Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.09.136
Keywords
Lactobacillus plantarum; Intestinal barrier; Strain specificity; Capsular polysaccharide
Funding
- National Natural Science Foundation of China
- Natural Sci-ence Foundation of Jiangsu Province
- [31820103010]
- [32122067]
- [BK20200084]
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This study investigated the strain-specific effects of 55 L. plantarum strains on the intestinal barrier. It found that the ability of L. plantarum to regulate the intestinal barrier is exerted in part by genes encoding proteins associated with polysaccharide synthesis. The study also identified different effects of L. plantarum strains on intestinal barrier dysfunction and suggested that the capsular polysaccharides of L. plantarum may be explored as a potential functional food component against intestinal barrier dysfunction.
The intestinal barrier is integral to the host's defense, and disrupting its integrity contributes to gut and systemic diseases. Lactobacillus plantarum has been widely reported to exhibit a protective effect on the gut barrier. However, the strain-specific mechanism of this bacterium's function remains unclear. This study characterized the regulative effects of 55 L. plantarum strains on the intestinal barrier using TNF-alpha-induced Caco-2 cells and a dextran sulfate sodium-induced colitis animal model and found that the regulative effect is strain-specific. Comparative genomic analysis suggested that the ability of L. plantarum to regulate the intestinal barrier is exerted in part by genes encoding proteins associated with polysaccharide synthesis. This observation was verified using surface protein/capsular polysaccharides separation experiments. Structural analysis of capsular polysaccharides showed that molecular weight and mole ratios of monosaccharide compositions may play important roles in strain-specific protective effects on the gut barrier. This study identified different effects of L. plantarum strains on intestinal barrier dysfunction and proved that this regulative ability relies on the charac-teristic of the capsular polysaccharides of the strains. Thus, our data provided genetic targets and molecular for screening L. plantarum strains with the ability to protect the gut barrier, and suggested the capsular poly-saccharides of L. plantarum may be explored as a potential functional food component against intestinal barrier dysfunction.
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