Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 222, Issue -, Pages 1127-1136Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.09.246
Keywords
Shenling Baizhu san polysaccharides; Gut microbiota; Inflammatory bowel disease
Funding
- National Natural Science Foundation of China [32072903, 31472232]
- R & D Projects in important areas of Guangdong Province, Studies and applications about key technology biosynthesis in antibiotic-free feeds [2019B020218003]
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In this study, it was found that Shenling Baizhu San polysaccharides (SP) effectively improved colitis in mice by regulating tryptophan metabolic function of their gut microbiota. This effect was dependent on the gut microbiota and could be transferred through fecal microbiota transplantation (FMT).
Shenling Baizhu San has beneficial effects on the metabolism of the gut microbiota, however, the mechanisms underlying microbiota metabolites mediated anti-inflammation signaling are not well understood. Previously, we have demonstrated that supplementation with Shenling Baizhu San alleviated antibiotic-associated diarrhea (AAD). The current study intends to investigate the dynamic modulation of Shenling Baizhu San polysaccharides (SP) on colitis from the gut microbiota metabolites perspective. Administration of SP effectively relieved colitis induced by DSS in mice, including alleviating body weight loss, the downregulation of colon proinflammatory mediators, and the promotion of intestinal injury repair. Whereas, the efficacy was eliminated by antibiotics, which demonstrated that the efficacy of SP was dependent on the gut microbiota. Fecal microbiota transplantation (FMT) showed that the efficacy of SP can be transferred to gut microbiota. Serum metabolomics analysis showed that supplementation with SP significantly promoted tryptophan metabolism, which was consistent with the changed structure of the gut microbiota, including Bacteroides, Bifidobacterium and Ruminococcus regulated by SP. Especially, the tryptophan metabolites-kynurenine (KYN) activated the expression of amplifying aryl-hydrocarbon receptor (AhR) and Cyp1A1 to promote IL-10 expression in colon. These data suggested that SP positively affected colitis in mice by regulating tryptophan metabolic function of their gut microbiota.
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