Journal
INORGANIC CHEMISTRY
Volume 61, Issue 50, Pages 20674-20689Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.inorgchem.2c03646
Keywords
-
Categories
Funding
- Labex ARCANE [ANR-17-EURE-0003]
- Agence Nationale de la Recherche [ANR-18-CE06-0022]
- Labex SynOrg [ANR-11-LABX- 0029]
- XL-Chem graduate school [ANR-18-EURE-0020]
- Region Normandie
- Agence Nationale de la Recherche (ANR) [ANR-18-CE06-0022] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
In this article, a novel microscopy imaging probe based on lanthanide complexes is described. The probe exhibits desirable luminescence properties and cell-penetrating ability for 2P imaging of live cells. By conjugating the lanthanide complexes with cell-penetrating peptides, successful synthesis of the conjugates was achieved and their application in various microscopy imaging techniques was evaluated. The results demonstrate that the probe has proper internalization and cytosolic delivery in cells, while maintaining its original luminescence properties. These findings provide new insights for the development of functional probes for live cell microscopy imaging.
Lanthanide(III) (Ln3+) complexes feature desirable luminescence properties for cell microscopy imaging, but cytosolic delivery of Ln3+ complexes and their use for 2P imaging of live cells are challenging. In this article, we describe the synthesis and spectroscopic characterizations of a series of Ln3+ complexes based on two ligands, L1 and L2, featuring extended picolinate push-pull antennas for longer wavelength absorption and 2P absorption properties as well as a free carboxylate function for conjugation to peptides. Several cell penetrating peptide/ Ln3+ complex conjugates were then prepared with the most interesting luminescent complexes, Tb(L1) and Eu(L2), and with two cell penetrating peptides (CPPs), ZF5.3 and TP2. A spectroscopic analysis demonstrates that the luminescence properties of the complexes are not affected by conjugation to the peptide. The conjugates were evaluated for one-photon (1P) time-gated microscopy imaging, which suppresses biological background fluorescence, and 2P confocal microscopy. Whereas TP2-based conjugates were unable to enter cells, successful 1P and 2P imaging was performed with ZF5.3[Tb(L1)]. 2P confocal imaging suggests proper internalization and cytosolic delivery as expected for this CPP. Noteworthy, 2P confocal microscopy also allowed characterization of the luminescence properties of the complex (spectrum, lifetime) within the cell, opening the way to functional luminescent probes for 2P confocal imaging of live cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available