Extraction of antioxidant and antimicrobial compounds from Inga marginata Willd bark and pulp using different extraction techniques and phytochemical characterization

This study aimed to identify the least aggressive and highest yield extraction method to obtain bioactive com-pounds from Inga marginata Willd fruits, determine the chemical components, and evaluate the extracts anti-microbial and cytotoxic activity. The extraction efficiency was expressed by the total phenolic and total flavonoid content and antioxidant activity (DPPH, IC50, and ORAC) using conventional, ultrasound, and microwave -assisted extraction. The highest bioactive compound content was achieved using 5 min at 60 degrees C for total phenolic content (214.98 mg GAE g-1), total flavonoid content (22.90 mg EQ g-1), DPPH (45.98 mu mol TEAC g- 1), inhibitory capacity (0.80 mg mL-1), and ORAC (167.25 mu mol Trolox g-1) using ultrasonic extraction, and the extract inhibited the growth of all microorganisms tested. Thirteen chemical compounds were determined by ESI-ToF-MS, confirming the high phytochemical capacity of the extract. Lastly, the Inga extract showed no cytotoxicity at the concentrations used.

Automated summary

This study aimed to find the optimal extraction method for obtaining bioactive compounds from Inga marginata Willd fruits, and evaluate their antimicrobial and cytotoxic activity. The extraction efficiency was measured by the total phenolic and total flavonoid content, as well as antioxidant activity using different methods. Ultrasonic extraction at 5 minutes and 60 degrees Celsius yielded the highest bioactive compound content, which showed inhibitory effects on the growth of microorganisms. Thirteen chemical compounds were identified, confirming the extract's high phytochemical capacity. Furthermore, the extract exhibited no cytotoxicity at the tested concentrations.