Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
Volume 59, Issue 1, Pages 121-128Publisher
SPRINGER
DOI: 10.1007/s11627-022-10322-4
Keywords
Corydalis saxicola; Leaf; Petiole; Adventitious shoot; pH value; Shoot proliferation; Regeneration; Rooting; Transplantation
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In this study, an efficient shoot proliferation and regeneration system for Corydalissaxicola Bunting was established for the first time. Shoot organogenesis from leaf and petiole explants was induced using cytokinins, and optimal growth conditions were identified for shoot proliferation and rooting.
Corydalissaxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi province of China. In this study, for the first time for this plant, an efficient adventitious shoot proliferation and regeneration system via shoot organogenesis from leaf and petiole explants was established. Cytokinins, including 6-benzyladenine (BA) and thidiazuron (TDZ), induced callus and then shoot organogenesis. Most adventitious shoots were induced from leaf explants on Murashige and Skoog (MS) medium (pH 6.5) supplemented with 2.0 mu M BA and 0.5 mu M alpha-naphthaleneacetic acid (NAA). This medium was also optimal for shoot proliferation. The rooting 1/2MS medium was supplemented with 1.0 mu M indole-3-butyric acid (IBA) and 0.2 mu M NAA; 95.7% of shoots induced roots within 30 d and 91% of plantlets survived on a peat:vermiculite (1:1, v:v) substrate after 90 d.
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