Structural characterization and biological activity of an a-glucan from the mollusk Marcia hiantina (Lamarck, 1818)

Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an alpha-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D H-1, H-1-H-1 COSY, H-1-H-1 TOCSY, H-1-H-1 NOESY, H-1-C-13 HSQC and H-1-C-13 HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived alpha-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of similar to 570 kDa. The mollusk alpha-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H2O2- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H2O2- and LPS-induced ROS, and potent cell proliferative activity.

Automated summary

Recent research has shown that edible clams, such as Marcia hiantina, contain polysaccharides with antioxidant, immunomodulatory, and antitumor activities. In this study, an alpha-glucan was isolated from M. hiantina and characterized. It was found to be a branched polysaccharide composed mainly of glucose, with the molecular weight of approximately 570 kDa. Cell-based assays demonstrated that the alpha-glucan had no cytotoxicity, exhibited potent antioxidant activity, and stimulated cell proliferation.