Role of siderophore in Pseudomonas fluorescens biofilm formation and spoilage potential function

We investigated the function of pyoverdine in the biofilm formation, motility, and spoilage potential of Pseu-domonas fluorescens. We targeted and identified two major genes (pvdA and pvdE) that are involved in the biosynthesis of siderophores. We next constructed Delta pvdA and Delta pvdE mutants of P. fluorescens PF08 and found that the deletion of pyoverdine led to a biofilm-to-motivity transition as both Delta pvdA and Delta pvdE mutants dis-played enhanced motility, reduced level of exopolysaccharides (EPSs), and attenuated biofilm formation. In addition, the lack of synthesis of pyoverdine promoted the spoilage of fish fillets stored at 4 degrees C. Based on the effect of pyoverdine deletion on the phenotype; we report that pyoverdine regulates the transcription levels of htpX, phoA, flip, flgA, and RpoS, suggesting that pyoverdine-mediated iron absorption may affect the regulation of flagellum and stress resistance. This study emphasizes the important role of pyoverdine in the formation of biofilm, motility, and spoilage of P. fluorescens PF08.

Automated summary

This study investigated the role of pyoverdine in the formation of biofilm, motility, and spoilage potential of P. fluorescens. The deletion of pyoverdine genes led to a transition from biofilm to motility and promoted spoilage of stored food. Pyoverdine was found to regulate the transcription levels of several genes involved in bacterial motility and stress response.