Release of bound polyphenols from wheat bran soluble dietary fiber during simulated gastrointestinal digestion and colonic fermentation in vitro

Dietary fiber can act as a carrier of bound phenolics in the distal tracts of the gut, where potential microbial processing occurs, but specific mechanism is unclear. This study aimed to evaluate the release characteristic and activity of bound polyphenols from wheat bran soluble dietary fiber (SDF) during simulated gastrointestinal digestion and colonic fermentation in vitro. The result suggested that the bioaccessibility of bound polyphenols in colonic fermentation was 7.42 times that in gastrointestinal digestion stage. Gallic acid, p-hydroxybenzoic acid and vanillic acid were the most abundant polyphenol metabolites after the fermentation for 6 h. The released phenolics exhibited strong radical scavenging activity (DPPH, 99.22 +/- 2.05 mu mol TE/100 g DW; ABTS, 330.27 +/- 3.56 mu mol TE/100 g DW). Fecal fermentation of SDF significantly reduced the value of F/B and stimulated the growth of beneficial bacteria, such as Bacteroides, Akkermansia, and Faecalibacterium. Therefore, bound poly -phenols may maintain gut health through their prebiotic activity.

Automated summary

Dietary fiber in wheat bran can act as a carrier of bound polyphenols in the gut, promoting gut health through their prebiotic activity and strong radical scavenging activity. Colonic fermentation of soluble dietary fiber significantly increases the bioaccessibility of bound polyphenols and stimulates the growth of beneficial bacteria while reducing the abundance of harmful bacteria.