Journal
FOOD CHEMISTRY
Volume 398, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133761
Keywords
AFB1; Aptamer; Yolk-shell; Fe3O4; Constitutional dynamic networks
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In this study, a fluorescent aptasensor using a new type of biomimetic network-amplification strategy was developed for the detection of aflatoxin B1 (AFB1). The aptasensor exhibited good sensitivity and detection limit in the low concentration range, and showed practical application value in the field of food safety.
In this study, yolk-shell Fe3O4-based nanomaterials designing of fluorescent aptasensor using a new type of biomimetic network-constitutional dynamic networks (CDNs) amplification strategy was developed for aflatoxin B1 (AFB1) detection. By ingeniously designing the base sequences A, a, B, b, and coupling with endonuclease (BbvCI), a constitutes of CDNs is formed as a fluorescence intensity amplifier. With such design, the as-prepared aptasensor exhibits good sensitivity from 50 fg & BULL;mL-1-50 ng & BULL;mL-1 with a detection limit of 35.94 fg & BULL;mL-1. Moreover, the CDNs can achieve 1000-fold amplification in terms of linear range and detection limit. The results confirmed that this new type of biomimetic network amplification strategy can supply-one efficient approach to improve signal amplification. Furthermore, the prepared aptamer sensor was tested for spiked recovery in peanut samples, and the recoveries ranged from 83.79 to 95.06 %, which has confirmed its practical application value in the field of food safety.
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