Circular RNA circPlce1 regulates innate immune response in miiuy croaker, Miichthys miiuy

In recent years, more and more researchers have devoted to the study of circular RNAs (circRNAs) in noncoding RNAs. As an important regulator in a variety of biological processes, circRNAs are relatively abundant in the study of mammals, while research in lower vertebrates is still lacking. In this study, we found a circRNA, circPlce1, related to innate immune response in Miichthys miiuy (miiuy croaker). The experimental results confirmed that circPlce1 could promote the production of antiviral genes and inflammatory response under the stimulation of poly (I: C) and LPS. We also confirmed that circPlce1 can promote NF-kappa B and IRF3 pathways through luciferase reporter assay experiment. In addition, we also found that circPlce1 can promote cell pro-liferation and improve cell viability. In conclusion, our results showed that circPlce1 plays an active role in regulating inflammatory response, cell proliferation and cell viability, providing a foundation for the study of the biological function of circRNAs in the innate immune response in teleost fish.

Automated summary

In recent years, there has been an increasing focus on the study of circular RNAs (circRNAs) in noncoding RNAs. While circRNAs are abundant in mammalian studies, research in lower vertebrates has been lacking. In this study, a circRNA called circPlce1 was found to be related to the innate immune response in Miichthys miiuy (miiuy croaker). Experimental results showed that circPlce1 could promote the production of antiviral genes and inflammatory response. It was also observed that circPlce1 can promote cell proliferation and improve cell viability. The study provides a foundation for understanding the biological function of circRNAs in the innate immune response in teleost fish.