Involvement of Nrf2 in the immune regulation of Litopenaeus vannamei against Vibrio harveyi infection

NF-E2-related factor-like-2 (Nrf2) is a transcription factor that belongs to the Cap'n'Collar transcription factor family and plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. However, its influence on Vibrio spp infection in L. vannamei remains uncertain. In this study, the effects of Nrf2 on the immune response in Vibrio spp infection was determined by RT-PCR and histopathological analysis. The results showed that RNAi of Nrf2 significantly decreased the expression of antioxidant-related genes (CAT, SOD and GST; p < 0.05), and significantly up-regulated inflammation-related genes (IMD, pro-PO, P38, Toll, Hsp70, NF kappa B and RAB6A; p < 0.05) and the apoptosis gene (caspase3). Under the infection of V. harveyi, histopatho-logical analysis showed that after RNAi of Nrf2, the hepatopancreas of shrimp has an abnormal arrangement of hepatic tubules and vacuolization of hepatocyte; The basement membrane is peeled off and the epithelial cells are massively necrotic. Compared with the RNAi of Nrf2 group, the tissue damage in the SFN group was much lessened, and there were fewer apoptosis signals in the TUNEL assay. In conclusion, this experiment indicated that Nrf2 is involved in the regulation of inflammatory response, oxidative stress,and apoptosis induced by V. harveyi in L. vannamei.

Automated summary

NF-E2-related factor-like-2 (Nrf2), a transcription factor belonging to the Cap'n'Collar transcription factor family, plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. The effects of Nrf2 on the immune response in Vibrio spp infection were investigated. The results showed that Nrf2 RNAi decreased antioxidant-related genes expression and up-regulated inflammation-related genes and apoptosis gene. Histopathological analysis revealed tissue damage in Nrf2 RNAi group, while SFN group exhibited lessened tissue damage and fewer apoptosis signals.