Journal
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 179, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ejps.2022.106305
Keywords
Biomimetic barrier model; Barrier integrity; Transepithelial electrical resistance (TEER); Apparent permeability; Epithelium
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Funding
- German Federal Ministry of Education and Research (BMBF)
- [16LW0133K]
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This study discusses the development of biomimetic in vitro lung models, focusing on two gold standard techniques for monitoring barrier integrity. The advantages, limitations, and possible future directions of these methods are discussed.
The development of biomimetic in vitro lung models as an alternative to animal studies is urgent to improve the predictability of the pharmacokinetics of potential new drugs. For pharmacokinetics studies, advanced in vitro lung models such as lung-chips should mimic a functional air-blood barrier. Unlike in vivo conditions, stem/ primary cells and cell lines do not necessarily form a functional and tight barrier when cultured in vitro. Here, we explore the two gold standard techniques for monitoring barrier integrity: transepithelial electrical resistance (TEER) and permeability. We discuss the advantages and limitations of these methods, provide recommendations for methodological improvements, and we elude on possible future directions.
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