Journal
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
Volume 43, Issue 1, Pages 50-56Publisher
WILEY-BLACKWELL
DOI: 10.1111/jog.13149
Keywords
hypoxia; placenta; thioredoxin binding protein-2 (TBP-2); thioredoxin interacting protein (Txnip)
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [26462487]
- Precursory Research for Embryonic Science and Technology (PRESTO) from Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [26462487, 24119004] Funding Source: KAKEN
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AimThioredoxin binding protein-2 (TBP-2), which is identical to thioredoxin interacting protein (Txnip), controls cellular proliferation and differentiation. The aim of the present study was to compare TBP-2 protein and mRNA expression in human placenta during the three trimesters of pregnancy and to investigate the role of hypoxia in the change of these expressions in placental tissue. A secondary objective was to determine the gene expression of peroxisome proliferator-activated receptors (PPARs) in TBP-2 deficient placenta using TBP-2 gene disrupted mice (TBP-2(-/-)). MethodsProtein and mRNA expression of TBP-2 in human placenta from each trimester were analyzed by immunohistochemistry, Western blots, and by quantitative reverse-transcriptase-polymerase chain reaction. The effect of hypoxia on TBP-2 expression was tested using an explant culture of human placenta. In TBP-2(-/-) mouse placenta, we detected PPAR mRNA expression. ResultsTBP-2 was located in syncytiotrophoblasts and cytotrophoblasts, and also in the endothelium in human placenta. Its expression in the placenta was low in the first trimester, and increased in the second and third trimesters. Hypoxia decreased TBP-2 mRNA and protein expression in human placental explant culture. In TBP-2(-/-) mice, placental mRNA levels of PPAR and were significantly suppressed compared with those in wild-type mice. ConclusionHypoxia suppresses TBP-2 gene expression, which may ultimately alter placental development.
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