Journal
ENVIRONMENTAL RESEARCH
Volume 215, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2022.114291
Keywords
Lignocellulosic biomass; Enzymatic saccharification; Lignin properties; Interaction mechanisms; Non -enzymatic proteins
Funding
- National Key Research and Develop- ment Program of China [2019YFE0114600, 2021YFE0114400]
- National Natural Science Foundation of China [52106245]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- 111 Project [111-2-06]
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This review discusses the use of non-catalytic additive proteins to enhance enzymatic hydrolysis (EH). It explores the various mechanical steps involved in the EH process and investigates the effects of modified lignin on EH, including its interaction with cellulase and cellulose. The review also explains the different conditions that can lead to cellulase deactivation. Overall, recent advances and potential mechanisms of non-catalytic proteins in EH are evaluated and future perspectives are presented.
Fermentable sugar production from lignocellulosic biomass has received considerable attention and has been dramatic progress recently. However, due to low enzymatic hydrolysis (EH) yields and rates, a high dosage of the costly enzyme is required, which is a bottleneck for commercial applications. Over the last decades, various strategies have been developed to reduce cellulase enzyme costs. The progress of the non-catalytic additive proteins in mitigating inhibition in EH is discussed in detail in this review. The low efficiency of EH is mostly due to soluble lignin compounds, insoluble lignin, and harsh thermal and mechanical conditions of the EH process. Adding non-catalytic proteins into the EH is considered a simple and efficient approach to boost hydrolysis yield. This review discussed the multiple mechanical steps involved in the EH process. The effect of physicochemical properties of modified lignin on EH and its interaction with cellulase and cellulose are identified and discussed, which include hydrogen bonding, hydrophobic, electrostatic, and cation-pi interactions, as well as physical barriers. Moreover, the effects of different conditions of EH that lead to cellulase deactivation by thermal and mechanical mechanisms are also explained. Finally, recent advances in the development, potential mechanisms, and economic feasibility of non-catalytic proteins on EH are evaluated and perspectives are presented.
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