Spatiotemporal distribution of opportunistic pathogens and microbial community in centralized rural drinking water: One year survey in China

Centralized water supply in rural areas, supported by small waterworks (small-central mode) and by municipal water treatment plants (urban-extension mode), is an important guarantee to implement the Rural Revitalization Strategy Plan (2018-2022) in China. Opportunistic pathogens (OPs) could not be evaluated by the national drinking water sanitation standards in China (GB 5749-2022), posing potential microbial risks in rural drinking water. In this study, the spatiotemporal distribution of OPs, microbial community and the associated functional composition under two central water supply modes were investigated by molecular approaches. The results indicated that OPs were widely presented in the rural drinking water regardless of water supply modes, and were more abundant than those in the urban tap water. The insufficient residual chlorine and higher turbidity triggered more microbial proliferation, posing a seasonal variation of OPs gene copy numbers and bacterial community compositions. In warm seasons of summer and autumn, the gene copies of E. coli, M. avium, Pseudomonas spp. and the amoeba host Acanthamoeba spp. achieved up to 4.92, 3.94, 6.75 and 3.74 log10 (gene copies/mL), respectively. Potential functional prediction indicated higher relative abundance of pathogenic genes and infectious risks associated with the rural drinking water under small-central water supply mode. This one-year survey of the spatiotemporal distribution of OPs and microbial community provided scientific insights into microbial safety of rural drinking water, prompting attention on small-central water supply mode against OPs risks.

Automated summary

Centralized water supply in rural areas is crucial for implementing the Rural Revitalization Strategy Plan in China. This study found that opportunistic pathogens were widely present in rural drinking water regardless of the water supply mode, and their abundance was higher than in urban tap water. The insufficient residual chlorine and higher turbidity in warm seasons triggered microbial proliferation and seasonal variation in opportunistic pathogens. The small-central water supply mode in rural areas had a higher relative abundance of pathogenic genes and infectious risks. This study provides important scientific insights into the microbial safety of rural drinking water and highlights the need to address opportunistic pathogens risks in small-central water supply mode.