Journal
ENVIRONMENTAL POLLUTION
Volume 323, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2023.121208
Keywords
Estrogen receptor; Endocrine disrupting chemical; Bivalve molluscs; Molecular docking; Recombinant yeast
Categories
Ask authors/readers for more resources
Endocrine disrupting chemicals (EDCs) in estuaries and coastal habitats have caused global concern. Bivalves were thought to be less sensitive to these substances compared to vertebrates, but a computational simulation revealed the weak activation ability of bivalve estrogen receptors (ERs) in response to steroids and EDCs. The presence of an active ER in bivalve molluscs was confirmed, providing a promising tool for screening marine environmental pollutants and understanding underlying mechanisms.
Endocrine disrupting chemicals (EDCs) in estuaries and coastal habitats have been widely detected over the world and caused global concern. Bivalves have been shown to be vulnerable to endocrine disruption. However, estrogen receptors (ERs) sensitivity to steroids and EDCs has long been considered to be restricted to vertebrates. In the present study, a computational simulation docking model was applied to qualitatively predict the binding behavior of two bivalve ERs to estradiol and compared the docking activity with zebra fish ERa. A novel reconstituted yeast system was constructed by using transcriptional activator GAL-4 consists of ER-expressing plasmid and ERE (estrogen responsive element)-containing plasmid. The assays showed that bivalve ER specif-ically activate transcription in response to tested steroids and EDCs, but the activation ability is weaker compared to zebra fish ERa. The results corroborate the presence of an active ER in bivalve molluscs and provide a promising tool for screening of marine environmental pollutants active in disturbing ERs of bivalves, as well as understanding the underlying mechanism across taxonomic groups and phyla.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available