Journal
ELECTROPHORESIS
Volume 44, Issue 7-8, Pages 646-655Publisher
WILEY
DOI: 10.1002/elps.202200230
Keywords
counterflow gradient focusing; electroosmotic flow; free-flow electrophoresis; peak dispersion; protein purification
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Free-flow electrophoresis (FFE) is used to continuously separate charged solutes from complex biological mixtures. A free-flow counterflow gradient focusing mechanism has been introduced to improve resolution and versatility. This work models the impact of electroosmotic flow on solute dispersion and presents preliminary experimental results for validation.
Free-flow electrophoresis (FFE) has the ability to continuously separate charged solutes from complex biological mixtures. Recently, a free-flow counterflow gradient focusing mechanism has been introduced to FFE, and it offers the potential for improved resolution and versatility. However, further investigation is needed to understand the solute dispersion at the focal position. Therefore, the goal of this work is to model the impact of electroosmotic flow, which is found to produce a pressure-driven backflow to maintain the fixed counterflow inputs. Like the counterflow, this backflow has a parabolic velocity profile that must be considered when predicting the concentration distribution of a given solute. After the model is established, preliminary experimental results are presented for a qualitative comparison. Results demonstrate a reasonable agreement at low applied voltages and provide a strong framework for future experimental validation.
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