An effective NIR fluorescent molecular tool to monitor cancer cell migration derived from inhibiting autophagy-induced cellular inflammation

Autophagy, as a cellular self-protection mechanism, is closely related to the body's inflammatory response and cancer cell migration. Studies have shown that changes in lysosomal polarity can serve as good indicators of intracellular autophagy and inflammation. It can be seen that there may be a certain relationship between the occurrence of cancer cell migration and the polarity of lysosomes. To this end, we developed a novel near -infrared lysosome polarity probe (NIR-TCF) to explore the relationship between the three deeply. Here, NIR-TCF has a strong ICT effect and high sensitivity to slight polarity changes in emission shifts at 720 nm. In addition, NIR-TCF has low toxicity and can image intracellular autophagy and the relationship between auto-phagy and inflammation clearly. More importantly, NIR-TCF was used to assess autophagy inhibition-induced inflammation-driven cancer cell migration firstly.

Automated summary

Autophagy is closely related to the inflammatory response and cancer cell migration, and changes in lysosomal polarity can serve as indicators of intracellular autophagy and inflammation. The development of a novel near-infrared lysosome polarity probe allows for clear imaging of intracellular autophagy and inflammation, and assessment of autophagy inhibition-induced inflammation-driven cancer cell migration.