Journal
DIABETES
Volume 72, Issue 2, Pages 261-274Publisher
AMER DIABETES ASSOC
DOI: 10.2337/db22-0521
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This article explores the early cellular processes of autoimmune type 1 diabetes in rats, and identifies the stress response of beta- and alpha-cells as well as their association with immune cells. These findings provide insights for the development of therapeutic and prevention strategies for T1D.
Identifying the early islet cellular processes of autoimmune type 1 diabetes (T1D) in humans is challenging given the absence of symptoms during this period and the inaccessibility of the pancreas for sampling. In this article, we study temporal events in pancreatic islets in LEW.1WR1 rats, in which autoimmune diabetes can be induced with virus infection, by performing transcriptional analysis of islets harvested during the prediabetic period. Single-cell RNA-sequencing and differential expression analyses of islets from prediabetic rats reveal subsets of beta- and alpha-cells under stress as evidenced by heightened expression, over time, of a transcriptional signature characterized by interferon-stimulated genes, chemokines including Cxcl10, major histocompatibility class I, and genes for the ubiquitin-proteasome system. Mononuclear phagocytes show increased expression of inflammatory markers. RNA-in situ hybridization of rat pancreatic tissue defines the spatial distribution of Cxcl10(+) beta- and alpha-cells and their association with CD8(+) T cell infiltration, a hallmark of insulitis and islet destruction. Our studies define early islet transcriptional events during immune cell recruitment to islets and reveal spatial associations between stressed beta- and alpha-cells and immune cells. Insights into such early processes can assist in the development of therapeutic and prevention strategies for T1D.
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