Parabacteroides faecalis sp. nov. Isolated from Swine Faeces

A Gram-negative, obligate anaerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated AGMB00274(T) was isolated from swine faeces. An 16S rRNA gene analysis indicated that strain AGMB00274(T) belonged to the genus Parabacteroides, with the highest similarity to Parabacteroides johnsonii (P. johnsonii) DSM 18315(T) (sequence similarity of 94.9%). The genome size of strain AGMB00274(T) was 4,308,683 bp, with a DNA G+C content of 42.5 mol%. The biochemical analysis of strain AGMB00274(T) showed that it was positive for gelatin hydrolysis and alpha-fucosidase, but negative for the acid production from D-glucose, D-mannitol, D-maltose, salicin, glycerol, D-cellobiose, D-mannose, D-melezitose, D-sorbitol, D-trehalose, and negative for alpha-arabinosidase, glutamic acid decarboxylase, and pyroglutamic acid arylamidase. The dominant cellular fatty acids (> 10%) of the isolate were anteiso-C-15: 0 (23.2%), iso-C-15: 0 (16.6%), C-18: 1 omega 9c (16.4%), summed feature 11 (iso-C-17: 0 3-OH and/or C-18: 2 DMA) (12.5%), and C-16: 0 (11.3%). The major respiratory quinones of strain AGMB00274(T) were MK-9 (55.4%) and MK-10 (44.6%). The major polar lipid was phosphatidylethanolamine. Based on phylogenetic, genetic, physiological, and chemotaxonomic analyses, as a novel species of the genus Parabacteroides, strain AGMB00274(T) was proposed with the name Parabacteroides faecalis sp. nov. The type strain used was AGMB00274(T) (= KCTC 25286(T) = GDMCC 1.2742(T)).

Automated summary

A Gram-negative, obligate anaerobic, non-motile, non-spore-forming, rod-shaped bacterial strain AGMB00274(T) was isolated from swine faeces. It belonged to the genus Parabacteroides, with the highest similarity to Parabacteroides johnsonii DSM 18315(T) (sequence similarity of 94.9%). The strain was proposed as a novel species named Parabacteroides faecalis sp. nov. with the type strain AGMB00274(T) (= KCTC 25286(T) = GDMCC 1.2742(T)).