Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 224, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2023.113197
Keywords
Wound dressing; Composite cell-laden fiber; Mesenchymal stem cells; Biotextiles; Antimicrobial
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In this study, a dressing made of composite fibers carrying mesenchymal stem cells (MSCs) and an antimicrobial agent was developed using a scalable sateen textile technique. The encapsulated MSCs in the dressing remained viable and maintained expression of wound repair factors for up to 28 days. The dressing showed enhanced healing properties with increased collagen synthesis and exhibited antimicrobial activity without negatively impacting the MSCs.
Cutaneous wounds with impaired healing such as diabetic ulcers and burns constitute major and rapidly growing threat to healthcare systems worldwide. Accelerating wound healing requires the delivery of biological factors that induce angiogenesis, support cellular proliferation, and modulate inflammation while minimizing infection. In this study, we engineered a dressing made by weaving of composite fibers (CFs) carrying mesenchymal stem cells (MSCs) and a model antibiotic using a scalable sateen textile technique. In this regard, two different sets of CFs carrying MSCs or an antimicrobial agent were used to generate a multifunctional dressing. According to cell viability and metabolic activity as CCK-8 and live/dead with qRT-PCR results, more than %90 the encapsulated MSCs remain viable for 28 days and their expression levels of the wound repair factors including ECM remod-eling, angiogenesis and immunomodulatory maintained in MSCs post dressing manufacturing for 14 days. Post 10 days culture of the dressing, MSCs within CFs had 10-fold higher collagen synthesis (p < 0.0001) determined by hydroxyproline assay which indicates the enhanced healing properties. According to in vitro antimicrobial activity results determined by disk diffusion and broth microdilution tests, the first day and the total amount of release gentamicin loaded dressing samples during the 28 days were higher than determined minimal inhibition concentration (MIC) values for S. aureus and K. pneumonia without negatively impacting the viability and functionality of encapsulated MSCs within the dressing. The dressing is also flexible and can conform to skin curvatures making the dressing suitable for the treatment of different skin injuries such as burns and diabetic ulcers.
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