Journal
CLINICAL IMMUNOLOGY
Volume 245, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2022.109162
Keywords
Complementarity determining regions; Kidney transplantation; T cells; B cells; Immune repertoire; High -throughput sequencing
Categories
Funding
- China Postdoctoral Science Foundation [2021M691239]
- Guangxi Natural Science Foundation [2019GXNSFBA245032]
- Innovation Project of Guangxi Graduate Education [JGY2021140]
- Guangxi Science and Technology Plan Project [Gui Ke AD20238021]
- Guilin Science Research and Technology Development Project [20210218-2, 20190218-5-5]
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This study investigates the dynamic changes in the BCR-H (TCR-beta) CDR3 repertoire of kidney transplant patients. Specific gene subfamilies and sequence combinations were identified at different time points during the transplantation process.
Purpose: The dynamic immunity of kidney transplant patients has not been fully elucidated. In this study, we explored the repertoire features of B/T cell receptor (BCR/TCR) of kidney transplant patients.Methods: Using combined multiplex PCR amplification and high-throughput sequencing technique, we analyzed the uremic patients' BCR H chain and TCR beta chain repertoire which obtained 1 day before kidney trans-plantation (PRE-1), 1 day and 7 day after kidney transplantation (POST-1 and POST-7).Results: Our analysis results showed the diversity of TCR beta CDR3 in POST-7 group was highest. In addition, there were specific skewed usage of TRBV gene subfamilies, and V-J combinations in different time points during kidney transplantation. Moreover, the overlap degrees of BCR-H (TCR-beta) CDR3 repertoire among each group were identified. Notably, the abundance of some TCR-beta CDR3 sequences changed regularly in the time point of kidney transplantation.Conclusions: The BCR-H (TCR-beta) CDR3 repertoire of kidney transplant patients changed dynamically.
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