Journal
CHEMISTRY OF MATERIALS
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.chemmater.2c03138
Keywords
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Funding
- National Natural Science Foundation of China
- Research Foundation for Distinguished Scholars of Qingdao Agricultural University
- Shandong Provincial Natural Science Foundation
- Shandong Province High-er Educational Program for Young Innovation Talents
- Special Foundation for Taishan Scholar of Shandong Province
- [22274086]
- [22277062]
- [22076090]
- [665-1120020]
- [ZR2019YQ23]
- [ZR2020ZD37]
- [tsqn202103093]
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This study presents nitrogen-doped carbon materials templated from g-C3N4 with NOX-like behaviors. The s-NC nanozyme exhibits excellent catalytic activity in a wide pH range and is coupled with natural dehydrogenases to form a nanozyme-enzyme hybridization model. This work not only develops metal-free NOX-mimicking nanozymes but also provides important inspiration for NAD+-mediated biosynthesis using artificial nanozymes.
Exploration of high-performance nanozymes for specific biomolecules is crucial but challenging for practical application. Nicotinamide adenine dinucleotide (NADH) oxidase can drive efficient regeneration of the oxidized coenzyme NAD+, which is of great significance for numerous NAD+-dependent biocatalytic pathways. However, issues including its instability and incompatibility with tandem enzymes in terms of pH, temperature, etc. remain to be addressed. Here, we report nitrogen-doped carbon materials templated from g-C3N4 with NOX-like behaviors. The model s-NC nanozyme displays excellent catalytic activity in a wide pH range of 5.0-10.0 and a remarkable specific activity of up to 3.2 U/mg, which is superior to those of the reported NOX mimics. Furthermore, s-NC is coupled with natural dehydro-genases to develop a nanozyme-enzyme hybridization model for biosynthesis. Notably, it can well meet the pH requirement of the dehydrogenase under alkaline medium. This work not only develops NOX-mimicking metal-free nanozymes but also has inspired significance toward NAD+-mediated biosynthesis using artificial nanozymes.
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