Journal
CHEMICO-BIOLOGICAL INTERACTIONS
Volume 368, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2022.110208
Keywords
Arsenic; NF -KB and P53 balance; hsa_circ_0005050; circRNA-protein complex; Cell apoptosis; Cell proliferation
Funding
- National Natural Science Foundation of China
- [82160607]
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This study explored the role of hsa_circ_0005050 in mediating cell apoptosis and proliferation. It was found that hsa_circ_0005050 was highly expressed in individuals exposed to arsenic and could regulate the p53 and NF-KB signaling pathways to affect cell survival and proliferation. Additionally, hsa_circ_0005050 was found to interact with the RNA binding protein ILF3, influencing each other's formation.
The regulatory network between arsenic, genes and signaling pathways has been reported in arsenic carcino-genesis. Studies on circRNA represent a growing field, but the extent to circRNA potential mechanisms remains poorly understood. So this study we explore the systematic function of hsa_circ_0005050 in mediating the cell apoptosis and proliferation. We demonstrated that hsa_circ_0005050 was highly expressed in subjects who are long-term exposed to arsenic, and could be induced by NaAs2O3 in A549 and 16HBE. Knockdown of hsa_-circ_0005050 promotes A549 cell viability, whereas exerts the opposite effects in 16HBE. Mechanistically, hsa_circ_0005050 regulates the p53 and NF -KB signaling pathway involved in the apoptosis and proliferation. And we found that hsa_circ_0005050 could directly bind to the RNA binding protein ILF3 and mutually influence each other's formation. Upon si-hsa_circ_0005050, ILF3 export to the cytoplasm resulting the formation of a ternary complex ILF3-p65-IKBA, breaks the balance of p53 and NF -KB pathway and induces A549 apoptosis and leads to 16HBE proliferation. As a result of these investigations, suggestions were identified for future research.
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