4.7 Article

Multiple effects driven by pulsed electric field to enhance the catalytic efficiency of the mussel-inspired proteolytic membrane in protein hydrolysis

Related references

Note: Only part of the references are listed.
Article Chemistry, Applied

Enzymological characteristics of pepsinogens and pepsins purified from lizardfish (Saurida micropectoralis) stomach

Sakonwat Kuepethkaew et al.

Summary: Three pepsinogens (PG-I, PG-II, and PG-III) were purified from lizardfish stomach using ammonium sulfate precipitation and chromatographic columns. These pepsinogens were converted to pepsins with different molecular weights at pH 2.0, showing optimal activities at pH 2.0-3.5 and 40-50 degrees C. The purified pepsins were effective in hydrolyzing various proteins, with potential applications in protein food processing.

FOOD CHEMISTRY (2022)

Review Chemistry, Applied

The interactions of wine polysaccharides with aroma compounds, tannins, and proteins, and their importance to winemaking

Hayden R. Jones-Moore et al.

Summary: Polysaccharides in wine play a crucial role in modulating astringency, aroma volatility, and foaming by interacting with other wine components. Their interactions with tannins and proteins affect the clarity and stability of finished wines, making them important for overall wine quality.

FOOD HYDROCOLLOIDS (2022)

Article Agriculture, Dairy & Animal Science

Kinetics of pepsin-induced hydrolysis and the coagulation of milk proteins

Mengxiao Yang et al.

Summary: This study investigated the effects of pH and pepsin concentration on the hydrolysis of kappa-casein and coagulation of casein micelles. The results showed that the hydrolysis rate increased with increasing pepsin concentration, was pH dependent, and reached a maximum at pH ~6.0. Increasing pepsin concentration and decreasing pH resulted in a shorter coagulation time, and the extent of kappa-casein hydrolysis required for coagulation was independent of pepsin concentration at a given pH.

JOURNAL OF DAIRY SCIENCE (2022)

Article Chemistry, Physical

pH-responsive and sustained release drug delivery system of BSA coated CDs-DOX

Qianqian Duan et al.

Summary: A novel pH-responsive drug delivery system based on carbon quantum dots (CDs) was proposed, with bovine serum albumin (BSA) introduced to prepare a stable drug delivery system BSA@CDs-DOX. The system showed enhanced stability and prolonged release profile, with significantly reduced drug side effects on normal cells and improved killing effect on cancerous cells. Excellent and sustained migration resistance was observed, indicating good application prospect in chemotherapy of tumors.

JOURNAL OF MOLECULAR STRUCTURE (2022)

Article Food Science & Technology

Characterization of native lactic acid bacteria from traditionally fermented chhurpi of Sikkim Himalayan region for the production of chhurpi cheese with enhanced antioxidant effect

Rounak Chourasia et al.

Summary: Lactic acid bacteria (LAB) were isolated and characterized from chhurpi, a traditionally produced cheese product of Sikkim Himalaya. Enterococcus durans and Lactobacillus delbrueckii were the main LAB identified. Chhurpi produced using Lactobacillus delbrueckii WS4 had higher yield, protein hydrolysis, and antioxidant activity. Gastrointestinal digestion increased the antioxidant activity of chhurpi, especially for chhurpi produced by using Lb. delbrueckii WS4.

LWT-FOOD SCIENCE AND TECHNOLOGY (2022)

Article Engineering, Chemical

Modelling of oligodextran production via an immobilized enzyme membrane reactor: Bioreaction-separation coupling mechanism

Xianhui Li et al.

Summary: The use of immobilized enzymatic membrane reactors (EMR) is effective in enhancing oligodextran production efficiency, but optimization of substrate concentration, enzyme loading, and membrane selectivity and permeability is crucial for achieving optimal process performance. Immobilized EMR is limited by enzyme concentration, while EMR with free enzymes outperforms by utilizing a larger reaction area.

SEPARATION AND PURIFICATION TECHNOLOGY (2022)

Article Engineering, Environmental

Efficient heavy metal sequestration from water by Mussel-inspired polystyrene conjugated with polyethyleneimine (PEI)

Wenyan Gao et al.

Summary: This study developed a simple and green method for immobilizing PEI and removing Pb(II) by polymerizing PDA. The hybrid adsorbents showed superior selectivity, rapid kinetics, and satisfactory sorption-regeneration property in Pb(II) removal. The method also proved to be efficient for removing various metals like Fe(III), Cu(II), and Ni(II), offering a new perspective for mitigating heavy metal contamination.

CHEMICAL ENGINEERING JOURNAL (2022)

Article Chemistry, Applied

Effect of pulsed electric field on soybean isoflavone glycosides hydrolysis by β-glucosidase: Investigation on enzyme characteristics and assisted reaction

Chengwen Lu et al.

Summary: This study aimed to enhance the enzymatic hydrolysis of soybean isoflavone glycosides (SIG) using pulsed electric field (PEF) technology. The activity, kinetics, thermodynamics, and structure of beta-glucosidase (beta-GLU) were evaluated after PEF treatment. The results showed that PEF treatment improved the activity and catalytic efficiency of beta-GLU, and caused structural changes. The optimal conditions for PEF treatment resulted in a 94.58% yield of isoflavone aglycones.

FOOD CHEMISTRY (2022)

Article Food Science & Technology

Exploring the activation mechanism of alcalase activity with pulsed electric field treatment: Effects on enzyme activity, spatial conformation, molecular dynamics simulation and molecular docking parameters

Yinli Li et al.

Summary: This study revealed the mechanism by which pulsed electric field treatment increases alcalase activity, including partial unfolding of the secondary structure and improvement of the tertiary structure.

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES (2022)

Article Biochemistry & Molecular Biology

Insights into the interaction between chitosan and pepsin by optical interferometry

Ning Ma et al.

Summary: This study investigated the interaction between chitosan and pepsin at different concentrations and pH values using silica colloidal crystal films and ordered porous layer interferometry. The results showed that the protonation of chitosan and the net charge carried by pepsin caused various degrees of electrostatic attraction under different pH values, and the rate and degree of hydrolysis were positively correlated with pepsin concentration.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Article Biochemistry & Molecular Biology

Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies

Shalu Aggarwal et al.

Summary: In this study, trypsin was effectively immobilized on the surface of ZnO/Chitosan nanocomposite through covalent attachment. The size, structure, surface morphology, and elemental composition of the nanoparticles and nanocomposite were studied. Optimal immobilization conditions and catalytic activity were investigated. The results showed improved efficiency and stability of the immobilized trypsin, suggesting its potential application in biotechnological processes.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Review Biochemistry & Molecular Biology

A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

Roberto Morellon-Sterling et al.

Summary: This article highlights the complexities of immobilizing pepsin and discusses potential methods and applications. The lack of primary amino groups on the enzyme's surface and its poor stability at alkaline pH values present challenges for immobilization. The review also proposes possibilities for achieving both covalent immobilization and stabilization of pepsin through multipoint covalent attachment.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Article Chemistry, Applied

Formation of protein-anthocyanin complex induced by grape skin extracts interacting with wheat gliadins: Multi-spectroscopy and molecular docking analysis

Ziqi Guo et al.

Summary: The interaction between grape skin anthocyanin extract and gliadin was investigated, and it was found that anthocyanins could effectively form complexes with gliadin and change its conformational properties.

FOOD CHEMISTRY (2022)

Article Biochemistry & Molecular Biology

Immobilization of papain on nitrogen-doped graphene quantum dots improves the enzymatic properties and makes it a biosensor for cystatin C

Farzaneh Mirzaei et al.

Summary: In this study, papain was immobilized on nitrogen-doped graphene quantum dots (NGQDs) to enhance its stability. The immobilized papain showed improved stability compared to free enzyme, especially in the presence of organic solvents. The immobilization also led to a decrease in the Km and Vmax values of the enzyme. Additionally, NGQD-Glu-Papain exhibited high sensitivity to cystatin C.

PROCESS BIOCHEMISTRY (2022)

Article Chemistry, Applied

Mechanism of trypsin activation by pulsed electric field treatment revealed based on chemical experiments and molecular dynamics simulations

Yinli Li et al.

Summary: This study investigates the mechanism of trypsin activation by pulsed electric field (PEF) treatment using chemical experiments and molecular dynamics simulations. The results reveal that PEF treatment improves the interaction between the enzyme and substrate by increasing molecular hydrogen bonds and solvent-accessible surface area, while decreasing rotation radius and random coil content.

FOOD CHEMISTRY (2022)

Article Chemistry, Applied

Different thermal treatments of highland barley kernel affect its flour physicochemical properties by structural modification of starch and protein

Haoran Wang et al.

Summary: In this study, the effects of superheated steam (SS) and traditional heating methods on Highland barley (HB) were compared. The results showed that SS treatment improved the physiochemical properties of HB, including reducing gelatinization enthalpy, increasing the degree of gelatinization, delaying starch retrogradation, and changing the secondary structure of proteins.

FOOD CHEMISTRY (2022)

Article Food Science & Technology

High pressure assisted extraction of proteins from wet biomass of Arthrospira platensis (spirulina) - A kinetic approach

Marianna Giannoglou et al.

Summary: The application of high-pressure technology for protein extraction from cyanobacteria was investigated, and optimum conditions were found to be using 300 MPa pressure for 10 minutes with deionized water or phosphate buffer as the medium.

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES (2022)

Article Engineering, Chemical

Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study

Hao Zhang et al.

Summary: In this study, a novel enzymatic packed bed membrane reactor (EPBMR) was developed by integrating the advantages of both packed bed reactor (PBR) and enzymatic membrane reactor (EMR), enabling efficient and stable bioconversions. The use of microspheres and alkaline cleaning mitigated membrane fouling, and the selection of proper ultrafiltration membranes successfully produced oligodextran with desired properties.

JOURNAL OF MEMBRANE SCIENCE (2022)

Article Engineering, Multidisciplinary

Degradation of phenolic inhibitors by laccase immobilized on tannic acid/ polyethylenimine modified magnetic nanoparticles

Shiqian Li et al.

Summary: Fe3O4 magnetic nanoparticles were functionalized with TA/PEI and covalently immobilized laccase for degradation of phenolic inhibitors in lignocellulosic hydrolysate. The immobilized laccase showed high activity, stability, and potential for practical applications.

RESULTS IN ENGINEERING (2022)

Article Engineering, Chemical

Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study

Hao Zhang et al.

Summary: A novel enzymatic packed bed membrane reactor (EPBMR) was developed by integrating advantages of packed bed reactor (PBR) and enzymatic membrane reactor (EMR). The use of microspheres enlarged the contact area between enzymes and substrates and reduced membrane fouling. The system exhibited a high sucrose conversion rate after 36 hours of continuous operation.

JOURNAL OF MEMBRANE SCIENCE (2022)

Article Chemistry, Physical

Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

MengYa Guo et al.

Summary: PEI is commonly used in CO2 adsorption but rarely in enzyme immobilization. In this study, FDH was immobilized on PEI modified surfaces, resulting in significantly improved catalytic performance, stability, and activity compared to free enzymes. Polydopamine/PEI-SiO2 attached FDH showed higher activity and stability than biocatalytic membrane.

CATALYSIS COMMUNICATIONS (2021)

Article Engineering, Environmental

Modular assembly of enzyme loaded nanoparticles in 3D hollow fiber electrode for electrochemical sensing

Huimin Wu et al.

Summary: A three-dimensional enzyme loading strategy was developed by modular assembly of enzyme loaded nanoparticles with porous conductive membranes. The use of physical entrapment improved enzymatic stability and sensor sensitivity. The electrode structure showed fast response, wide linear range, high sensitivity, and low detection limit, making it promising for cancer cell metabolism monitoring and multi-target array detection.

CHEMICAL ENGINEERING JOURNAL (2021)

Article Engineering, Environmental

Enzyme-immobilized flat-sheet membrane contactor for green carbon capture

Hannaneh Rasouli et al.

Summary: The development of a biocatalytic flat sheet membrane for CO2 capture in membrane contactors shows promising performance and stability in industrial applications, highlighting its potential as an environmentally friendly approach in the field.

CHEMICAL ENGINEERING JOURNAL (2021)

Article Food Science & Technology

Effect of moderate electric field on structural and thermo-physical properties of sunflower protein and sodium caseinate

Busra Gultekin Subasi et al.

Summary: Moderate electric field treatment altered the structural and thermo-physical properties of sunflower protein isolate and sodium caseinate, leading to changes in particle size, surface tension, and helix content. The effects were more significant for SPI, resulting in a bulkier, loosened, and more hydrophilic structure, while changes in CAS were less pronounced. Denaturation temperatures and enthalpies of both SPI and CAS samples decreased after MEF treatments.

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES (2021)

Article Engineering, Chemical

Co-deposition of hyperbranched polyethyleneimine and dopamine on anion exchange membrane for improved antifouling performance

Lan Hao et al.

Summary: This study demonstrates a novel strategy of utilizing hyperbranched PEI and dopamine to design antifouling AEMs, which successfully enhances the membrane materials' fouling resistance while maintaining desalination performance.

JOURNAL OF MEMBRANE SCIENCE (2021)

Article Chemistry, Physical

Investigation on the interaction behavior between safranal and pepsin by spectral and MD simulation studies

Nazanin Dehdasht-Heidari et al.

Summary: This study investigates the interaction between safranal and pepsin under physiological conditions, revealing that safranal can alter the structure and function of pepsin, with non-covalent reactions playing a critical role in this interaction.

JOURNAL OF MOLECULAR LIQUIDS (2021)

Article Food Science & Technology

Combining Alcalase hydrolysis and transglutaminase-cross-linking improved bitterness and techno-functional properties of hypoallergenic soybean protein hydrolysates through structural modifications

Qiaozhi Zhang et al.

Summary: Combining controlled Alcalase hydrolysis with TGase-crosslinking effectively produces hypoallergenic soybean protein hydrolysates with improved flavor and functional properties. TGase treatment significantly reduces bitterness and enhances overall flavor of the hydrolysates while also improving emulsifying and foaming properties. The addition of TGase does not generate new allergens and does not distinctly affect the residual allergenicity of the hydrolysates.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

Article Biochemical Research Methods

Dopamine-polyethyleneimine co-deposition cellulose filter paper for α-Glucosidase immobilization and enzyme inhibitor screening

Peng Li et al.

Summary: In this study, cellulose filter paper was used as a carrier for immobilized alpha-glucosidase, showing that the immobilized enzyme maintains good activity and stability even after repeated use. The method proved to be effective for screening potential alpha-glucosidase inhibitors from Traditional Chinese medicines.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2021)

Article Agriculture, Dairy & Animal Science

Osteogenic effects of the peptide fraction derived from pepsin-hydrolyzed bovine lactoferrin

P. Wen et al.

Summary: Lactoferrin is a protein found in milk that shows osteogenic activity with good biocompatibility and tolerability, potentially improving osteoporosis. By hydrolyzing and separating lactoferrin, a highly effective bioactive component was isolated and a stimulating peptide segment P1 was successfully synthesized for promoting bone formation.

JOURNAL OF DAIRY SCIENCE (2021)

Review Environmental Sciences

Highly effective enzymes immobilization on ceramics: Requirements for supports and enzymes

Joanna Kujawa et al.

Summary: The article summarizes the methods and key elements of enzyme immobilization, including the selection and properties of supports, the impact of modifiers and crosslinkers on enzymes, and the functional groups of immobilized enzymes. By optimizing these factors, efficient immobilization of enzymes and good stability can be achieved.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Engineering, Chemical

Bioinspired proteolytic membrane (BPM) with bilayer pepsin structure for protein hydrolysis

Zhe Chen et al.

Summary: Peptides derived from food proteins have great potential as natural health products. The bioinspired proteolytic membrane (BPM) with bilayer pepsin structure was fabricated to produce peptides, showing improved enzyme loading, activity, and hydrolysis degrees. BR-BPM demonstrated higher enzyme loading, degree of hydrolysis, and permeate flux than other structures under various operation conditions, indicating promising application for peptides production.

SEPARATION AND PURIFICATION TECHNOLOGY (2021)

Article Engineering, Environmental

Superporous neutral, anionic, and cationic cryogel reactors to improved enzymatic activity and stability of α-Glucosidase enzyme via entrapment method

Sahin Demirci et al.

Summary: In this study, alpha-Glu enzyme was immobilized in different synthetic cryogels to enhance its stability and durability. The cryogels maintained high enzymatic activity under optimal conditions and showed varying activity levels at different pH values. Entrapment of alpha-Glu enzyme in cryogels resulted in improved operational and storage stability compared to free enzyme.

CHEMICAL ENGINEERING JOURNAL (2021)

Article Engineering, Chemical

Fabrication of a charged PDA/PEI/Al 2 O 3 composite nanofiltration membrane for desalination at high temperatures

Chen Zhao et al.

Summary: This study successfully synthesized composite ceramic nanofiltration membranes by co-deposition of polydopamine and polyethyleneimine, achieving a significant improvement in salt rejection. The composite membranes, with high mechanical stabilities and good hydrophilicity, can still effectively filter salt solutions at high temperatures.

SEPARATION AND PURIFICATION TECHNOLOGY (2021)

Article Chemistry, Applied

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

C. E. Klostermann et al.

Summary: The research indicates that crystal type has the largest influence on the resistance to digestion of RS-3 (A >>> B), followed by molecular weight (high DP >> low DP) and molecular weight distribution (narrow disperse > polydisperse). B-type crystals that escaped digestion showed changes in molecular weight and distribution, while A-type crystals remained unchanged, suggesting that pancreatic alpha-amylase binds and acts differently to A- or B-type RS-3 crystals.

CARBOHYDRATE POLYMERS (2021)

Article Biophysics

Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI

Tongle Liu et al.

Summary: By modifying graphene oxide with PDA/PEI and utilizing the affinity between metal ions and AC's His-tags, directional immobilization of AC was successfully achieved. The modified GO increases amino density for affinity group decoration and adjusts the interaction between AC and the support. This method can improve enzyme activity and immobilization efficiency.

COLLOIDS AND SURFACES B-BIOINTERFACES (2021)

Article Acoustics

Complexation of maltodextrin-based inulin and green tea polyphenols via different ultrasonic pretreatment

Shuyi Li et al.

Summary: The study showed that high-intensity ultrasound treatment could accelerate the interaction between inulin and tea polyphenols, leading to the formation of complexes. Spectral analysis confirmed the formation of hydrogen bonds between saccharides and phenols.

ULTRASONICS SONOCHEMISTRY (2021)

Article Engineering, Chemical

Nanostructured Polyphenol-Mediated Coating: a Versatile Platform for Enzyme Immobilization and Micropollutant Removal

Fangfang Zhou et al.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2020)

Article Chemistry, Analytical

Covalent immobilization of trypsin on polyvinyl alcohol-coated magnetic nanoparticles activated with glutaraldehyde

Selmihan Sahin et al.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2020)

Review Biotechnology & Applied Microbiology

Biocatalytic membrane: Go far beyond enzyme immobilization

Jianquan Luo et al.

ENGINEERING IN LIFE SCIENCES (2020)

Article Food Science & Technology

Electrical systems for pulsed electric field applications in the food industry: An engineering perspective

Rai Naveed Arshad et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2020)

Article Engineering, Chemical

Aggregation of oil droplets and demulsification performance of oil-in-water emulsion in bidirectional pulsed electric field

Boping Ren et al.

SEPARATION AND PURIFICATION TECHNOLOGY (2019)

Review Chemistry, Applied

Immobilization of Enzymes in/on Membranes and their Applications

Yu-Ke Cen et al.

ADVANCED SYNTHESIS & CATALYSIS (2019)

Article Engineering, Environmental

Fe3O4@MoS2@PEI-facilitated enzyme tethering for efficient removal of persistent organic pollutants in water

Fanpeng Ran et al.

CHEMICAL ENGINEERING JOURNAL (2019)

Article Engineering, Environmental

Mussel-inspired co-deposition to enhance bisphenol A removal in a bifacial enzymatic membrane reactor

Xiaotong Cao et al.

CHEMICAL ENGINEERING JOURNAL (2018)

Article Engineering, Chemical

Mussel-inspired modification of ion exchange membrane for monovalent separation

Jian Li et al.

JOURNAL OF MEMBRANE SCIENCE (2018)

Article Engineering, Chemical

Aflatoxin B1 removal by multifunctional membrane based on polydopamine intermediate layer

Sushuang Li et al.

SEPARATION AND PURIFICATION TECHNOLOGY (2018)

Review Food Science & Technology

Feasibility of using pulsed electric fields to modify biomacromolecules: A review

Stephen Gitonga Giteru et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2018)

Article Engineering, Chemical

Enhancing the activity of pectinase using pulsed electric field (PEF) treatment

Fusheng Zhang et al.

JOURNAL OF FOOD ENGINEERING (2017)

Article Biochemistry & Molecular Biology

Effects of Pulsed Electric Field (PEF) Treatment on Enhancing Activity and Conformation of α-Amylase

Mei-ling Tian et al.

PROTEIN JOURNAL (2016)

Article Biochemistry & Molecular Biology

Effect of the external electric field on selected tripeptides

Jozef Mazurkiewicz et al.

AMINO ACIDS (2015)

Article Biotechnology & Applied Microbiology

Pepsin immobilization on an aldehyde-modified polymethacrylate monolith and its application for protein analysis

Wenjuan Han et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2015)

Article Biochemistry & Molecular Biology

Extraction, partial purification and characterization of polyphenol oxidase from Solanum lycocarpum fruits

Karla A. Batista et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

Article Acoustics

Influence of low ultrasound intensity on the degradation of dextran catalyzed by dextranase

Mohanad Bashari et al.

ULTRASONICS SONOCHEMISTRY (2013)

Article Chemistry, Applied

Purification of chitosan by using sol-gel immobilized pepsin deproteinization

Bo Li et al.

CARBOHYDRATE POLYMERS (2012)

Article Biochemistry & Molecular Biology

Enzyme immobilization on carbon nanomaterials: Loading density investigation and zeta potential analysis

Kyoungseon Min et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)

Review Agriculture, Dairy & Animal Science

Characteristics of rennet and other enzymes from small ruminants used in cheese production

Ekaterini Moschopoulou

SMALL RUMINANT RESEARCH (2011)

Meeting Abstract Biotechnology & Applied Microbiology

Immobilization of chemically modified papain on cotton fabric

Yong Xue et al.

JOURNAL OF BIOTECHNOLOGY (2008)

Article Engineering, Electrical & Electronic

Influence of pulsed electric field on various enzyme activities

Takayuki Ohshima et al.

JOURNAL OF ELECTROSTATICS (2007)