Journal
CHEMICAL ENGINEERING JOURNAL
Volume 450, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2022.138362
Keywords
ECL biosensor; Binary luminophore; Boron carbon oxynitride; Breast cancer
Categories
Funding
- National Natural Science Foundation of China [82072378, 81601856]
- Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]
- Ba Yu Scholar Program, China [YS2019020]
- Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]
- Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China
- Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]
- Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China [cstc2014kjrc-qnrc00004]
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In this study, a high-efficient and low-cost electrochemiluminescence biosensor was developed for ultrasensitive detection of breast cancer related protein. The sensor utilized a novel luminophore and coreactant, showing promising detection performance and potential clinical applications.
For the first time, a high-efficient and low-cost electrochemiluminescence (ECL) biosensor was constructed for ultrasensitive detection of breast cancer related human epidermal growth factor receptor-2 (HER-2) using a novel binary luminophore and low concentration of coreactant. Firstly, Au nanoparticles decorated graphite-like carbon nitride (g-C3N4@Au) was designed as sensing platform, which can generate ECL signal in the potential range from -1.2 V to 0 V when peroxydisulfate (S2O82-) was used as the coreactant. Secondly, amino-modified capture probes (CP) were immobilized on the surface of g-C3N4@Au via Au-N bonds. In addition, Zr-based metal organic framework (Zr-MOF) modified bulk boron carbon oxynitride (BCNO), named ZMB, exhibited remarkable ECL intensity at the potential window of -2.0 V to 0 V with 0.8 mM S2O82-. ZMB possessed a large specific surface area and abundant amino groups to anchor Au@Pt nanoparticles (Au@PtNPs). Therefore, HER-2 binding aptamers (HBA) could be anchored on Au@PtNPs to form the tracer label. Under optimal conditions, the proposed ECL biosensor displayed a wide linear range from 10 fg mL(-1) to 100 ng mL(-1) with a limit of detection (LOD) as low as 0.2 fg mL(-1). Importantly, this work utilized ZMB/S2O82- as new ECL system with satisfactory detection performances, which provided a potential approach to improve the sensitivity in clinical diagnosis.
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