4.4 Review

Mechanistic Insights into the Activities of Major Families of Enzymes in Bacterial Peptidoglycan Assembly and Breakdown

Related references

Note: Only part of the references are listed.
Review Biochemistry & Molecular Biology

LD-transpeptidases: the great unknown among the peptidoglycan cross-linkers

Alena Aliashkevich et al.

Summary: The peptidoglycan (PG) cell wall is crucial for bacterial shape and viability, with protective functions due to its mesh-like structure. Penicillin-binding proteins (PBPs) are important targets for antibiotics, while LD-transpeptidases (LDTs) have been less studied but are now recognized for their role in cell wall adaptation to stress, outer membrane stability, and toxin delivery in some bacteria. Further research is needed to fully understand the biological significance of LDTs and their potential as main PG-transpeptidases in certain species.

FEBS JOURNAL (2022)

Review Chemistry, Multidisciplinary

Recent Advances in the Synthesis and Biological Applications of Peptidoglycan Fragments

Condurache M. Vacariu et al.

Summary: The biosynthesis, breakdown, and modification of peptidoglycan (PG) are crucial for bacterial viability and human response to bacterial infection. Chemical synthesis is an important method to obtain PG fragments that can be used as enzyme substrates and inducers of the human immune response. This review highlights recent advancements in the synthesis and biochemical studies of PG fragments, PG biosynthetic intermediates, and PG breakdown products.

CHEMISTRY-A EUROPEAN JOURNAL (2022)

Article Biochemistry & Molecular Biology

Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis

Veronique Roig-Zamboni et al.

Summary: The cleavage of septal peptidoglycan during cell division helps in separating the daughter cells. The N-acetylglucosaminidase AtlA in Enterococcus faecalis plays a crucial role in cell separation and its mutants show decreased virulence. AtlA has structural homologs in other pathogens, making it a potential target for developing inhibitors of bacterial pathogenesis.

JOURNAL OF BIOLOGICAL CHEMISTRY (2022)

Article Microbiology

A Cell Wall Hydrolase MepH Is Negatively Regulated by Proteolysis Involving Prc and NlpI in Escherichia coli

Wook-Jong Jeon et al.

Summary: "The assembly of cell walls in Gram-negative bacteria is dependent on the activity of DD-endopeptidases, which cleave peptidoglycan crosslinks and need to be tightly regulated for maintaining cell wall integrity during expansion. This study discovered that MepS and MepM, two major DD-endopeptidases in Escherichia coli, are negatively controlled by the periplasmic protease Prc. Through genetic selections in rich medium, the authors identified suppressor mutations in prc and nlpI genes, and demonstrated that MepH is required for the suppression of growth defects caused by MepS(-) MepM(-) mutants. Furthermore, the study revealed that MepH is directly degraded by Prc and this degradation is enhanced by NlpI. Therefore, it concluded that all three major DD-endopeptidases involved in peptidoglycan assembly in E. coli are regulated by a common periplasmic protease."

FRONTIERS IN MICROBIOLOGY (2022)

Review Microbiology

Messengers From the Gut: Gut Microbiota-Derived Metabolites on Host Regulation

Chenyu Li et al.

Summary: The gut microbiota plays a crucial role in maintaining host health by producing metabolites that influence host functions. Understanding the structures and actions of gut microbiota-derived metabolites is important for studying microbiota-host interactions.

FRONTIERS IN MICROBIOLOGY (2022)

Article Biochemistry & Molecular Biology

Chemoenzymatic Probes Reveal Peptidoglycan Recognition and Uptake Mechanisms in Candida albicans

Lanxin Li et al.

Summary: The mechanisms of how Candida albicans recognizes and uptakes bacterial peptidoglycan fragments have not been well elucidated. By creating peptidoglycan probes, it was shown that natural peptidoglycan fragments interact with the Cyr1 sensor protein of C. albicans and enter the cells through an energy-dependent endocytic process.

ACS CHEMICAL BIOLOGY (2022)

Article Multidisciplinary Sciences

Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation

Julia E. Page et al.

Summary: Construction and remodeling of the bacterial peptidoglycan cell wall is essential for cell growth and division. Hydrolases, such as LytH in Staphylococcus aureus, play a crucial role in this process and can be potential targets for new antibiotics. The protein partner, ActH, is required for LytH activity and its amidase-activating function is found in its extracellular tetratricopeptide repeats (TPRs). Our findings suggest that metal cofactor stabilization is a general strategy used by amidase activators and ActH functions as a multi-functional protein.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2022)

Article Multidisciplinary Sciences

Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine

Yang Xu et al.

Summary: In this study, a method for fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine (GlcNAc) was reported. Uridine diphosphate N-azidoacetylglucosamine (UDP-GlcNAz) was successfully synthesized using the glycosyl-transferase OleD, and the fluorescent GlcNAz-labeled Lipid II was incorporated into the peptidoglycan through DD-transpeptidase activity of bifunctional class A penicillin-binding proteins.

ISCIENCE (2022)

Article Microbiology

Gut microbial DL-endopeptidase alleviates Crohn's disease via the NOD2 pathway

Jie Gao et al.

Summary: This study reveals that the depletion of DL-endopeptidase contributes to the pathogenesis of Crohn's disease through NOD2 signaling, providing a potential therapeutic target.

CELL HOST & MICROBE (2022)

Review Biochemistry & Molecular Biology

Imitate to illuminate: labeling of bacterial peptidoglycan with fluorescent and bio-orthogonal stem peptide-mimicking probes

Huibin Lin et al.

Summary: This article reviews the recent advances in the use of peptide-based probes for studying bacterial peptidoglycan (PGN). These probes, with their design rationale and functioning mechanisms, enable the direct evaluation of PGN presence and specific steps in PGN biosynthesis in microbial systems. The limitations and future development of these tools are also discussed.

RSC CHEMICAL BIOLOGY (2022)

Review Microbiology

Uptake, recognition and responses to peptidoglycan in the mammalian host

Paulo A. D. Bastos et al.

Summary: The microbiota and their signaling molecules play a significant role in influencing various physiological and behavioral consequences in the host organism. Peptidoglycan, a major component of bacterial cell surface, is released from gut bacteria, crosses the gut barrier, and enters the host system, influencing a range of physiological processes in the host. The molecular nature of peptidoglycan, the cellular profile of the recipient tissue, and the local immune context all play a role in determining the outcome for the host.

FEMS MICROBIOLOGY REVIEWS (2021)

Article Chemistry, Multidisciplinary

Synthesis of Carbapenems Containing Peptidoglycan Mimetics and Inhibition of the Cross-Linking Activity of a Transpeptidase of l,d Specificity

Saidbakhrom Saidjalolov et al.

Summary: Carbapenems have been optimized to target Gram-negative bacteria producing extended-spectrum beta-lactamases for the treatment of tuberculosis. The introduction of substituents at position C2 has shown to increase drug efficacy. Synthesizing peptido-carbapenems with a phenethylthio substituent at C2 demonstrated improved activity compared to the reference drug meropenem.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Article Biochemistry & Molecular Biology

MltG activity antagonizes cell wall synthesis by both types of peptidoglycan polymerases in Escherichia coli

Jessica L. Bohrhunter et al.

Summary: The peptidoglycan (PG) cell wall surrounding bacterial cells is crucial for cell integrity and is a key antibiotic target. MltG has been identified as a potential terminase for PG synthesis in Escherichia coli, acting by cleaving PG glycans during active synthesis.

MOLECULAR MICROBIOLOGY (2021)

Article Microbiology

Direct and Indirect Interactions Promote Complexes of the Lipoprotein LbcA, the CtpA Protease and Its Substrates, and Other Cell Wall Proteins in Pseudomonas aeruginosa

Dolonchapa Chakraborty et al.

Summary: The lipoprotein LbcA in Pseudomonas aeruginosa promotes the activity of the protease CtpA by recruiting the protease and its substrates independently, playing a crucial role in proteolysis. The copurification partners of LbcA are enriched for cell wall-associated proteins, with some being novel CtpA substrates. Additionally, some copurification partners not cleaved by CtpA may participate in multienzyme complexes containing CtpA substrates. These findings are significant for understanding CTPs and their associated proteins in peptidoglycan remodeling and virulence in various bacterial species.

JOURNAL OF BACTERIOLOGY (2021)

Article Microbiology

CwlQ Is Required for Swarming Motility but Not Flagellar Assembly in Bacillus subtilis

Sandra Sanchez et al.

Summary: This study identifies the lytic transglycosylase CwlQ as a critical enzyme for flagellum-dependent swarming motility in Gram-negative bacteria, while the lyase essential for flagellar insertion in Gram-positive bacteria remains unknown. Even when mutated in combination with other lyases related to motility, CwlQ mutation does not affect flagellar biosynthesis in Gram-positive bacteria.

JOURNAL OF BACTERIOLOGY (2021)

Article Biochemistry & Molecular Biology

The exo-β-N-acetylmuramidase NamZ from Bacillus subtilis is the founding member of a family of exo-lytic peptidoglycan hexosaminidases

Maraike Mueller et al.

Summary: Research has identified that the ybbC gene in Bacillus subtilis encodes a novel exo-beta-N-acetylmuramidase named NamZ, which plays a crucial role in cell wall turnover and recycling. NamZ is able to sequentially hydrolyze intact peptidoglycan from the non-reducing ends and belongs to a new family of glycosidases.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Biochemistry & Molecular Biology

The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment

Elizabeth A. Mueller et al.

Summary: The study reveals changes in the activity of two amidases, AmiB and AmiC, in E. coli under growth medium-related conditions, with the stimulation mediated through periplasmic amidase activators.

MOLECULAR MICROBIOLOGY (2021)

Article Biochemistry & Molecular Biology

ActS activates peptidoglycan amidases during outer membrane stress in Escherichia coli

Carlos K. Gurnani Serrano et al.

Summary: In this study, it was shown that the morphological defects and lysis associated with a ldtF mutant with impaired LPS transport can be alleviated by the loss of the OM-anchored lipoprotein ActS. ActS was found to be an inactive member of LytM-type peptidoglycan endopeptidases, capable of activating several periplasmic peptidoglycan amidases. Additionally, ActS was shown to preferentially activate AmiC and its function is linked to cell envelope stress.

MOLECULAR MICROBIOLOGY (2021)

Article Biology

Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins

Victor M. Hernandez-Rocamora et al.

Summary: Peptidoglycan is a crucial part of the bacterial cell envelope, and the Class A penicillin-binding proteins are essential enzymes involved in its synthesis. Researchers have developed a new assay to study the mechanisms of these enzymes in a lipid-bilayer environment, opening up possibilities for new directions in peptidoglycan synthesis research.

ELIFE (2021)

Article Genetics & Heredity

A lipoprotein allosterically activates the CwID amidase during Clostridioides difficile spore formation

Carolina Alves Feliciano et al.

Summary: Spore-forming pathogens such as Clostridioides difficile rely on germination to cause infection, where the production of malamycin (MAL) is crucial for cortex degradation. The binding of GerS lipoprotein to CwID amidase not only promotes the stability of Zn2+ co-factor binding, but also reveals a novel mechanism for regulating bacterial amidase activity. This study suggests that allosteric regulation by binding partners may be more common in bacterial amidase regulation than previously understood.

PLOS GENETICS (2021)

Article Multidisciplinary Sciences

Cleavage of Braun's lipoprotein Lpp from the bacterial peptidoglycan by a paralog of L,D-transpeptidases, LdtF

Raj Bahadur et al.

Summary: This study reveals the discovery of LdtF, a murein endopeptidase with previously unknown catalytic specificity that removes the PG-Lpp cross-links. The findings suggest a role for LdtF in regulating PG-OM linkages to maintain the structural integrity of the bacterial cell envelope.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Microbiology

DpaA Detaches Braun's Lipoprotein from Peptidoglycan

Matthias Winkle et al.

Summary: Research on E. coli has revealed that the attachment of Lpp to peptidoglycan in Gram-negative bacteria is a dynamic process, where detachment of Lpp can be beneficial under certain stress conditions. Furthermore, the enzyme DpaA, identified in this study, is present in many Gram-negative bacteria and may have different substrates in these species.
Article Multidisciplinary Sciences

A peptidoglycan storm caused by β-lactam antibiotic's action on host microbiota drives Candida albicans infection

Chew Teng Tan et al.

Summary: Broad-spectrum antibiotics can induce the release of peptidoglycan fragments by commensal bacteria, leading to the invasive growth of C. albicans and systemic dissemination, which is a significant risk factor for infection.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

CryoEM structure of the antibacterial target PBP1b at 3.3Å resolution

Nathanael A. Caveney et al.

Summary: The structural understanding of class A penicillin binding proteins is incomplete due to the difficulty in their crystallization and the complexity of their substrates. In this study, the structure of the 83kDa class A PBP from Escherichia coli, PBP1b, was successfully determined using cryogenic electron microscopy and a membrane mimetic, paving the way for structure-guided antibiotic development.

NATURE COMMUNICATIONS (2021)

Article Genetics & Heredity

Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexes

Ying Li et al.

Summary: This study elucidates the activation mechanism of the FtsW and FtsI complex during bacterial cell division, revealing that the activation signal triggered by FtsN passes through FtsQLB to FtsI and then to FtsW. By mapping mutations affecting FtsWI activation to the interaction interface structure, it is suggested that the interaction between SEDS proteins and their cognate bPBPs plays a critical role in the activation mechanism.

PLOS GENETICS (2021)

Article Biochemistry & Molecular Biology

Role of endopeptidases in peptidoglycan synthesis mediated by alternative cross-linking enzymes in Escherichia coli

Henri Voedts et al.

Summary: Bacteria resist turgor pressure through peptidoglycan, with endopeptidases playing a crucial role in the synthesis of cell wall, facilitating the formation of different cross-linking structures.

EMBO JOURNAL (2021)

Article Multidisciplinary Sciences

Biochemical reconstitution defines new functions for membrane-bound glycosidases in assembly of the bacterial cell wall

Atsushi Taguchi et al.

Summary: The peptidoglycan cell wall is essential for the survival of bacteria. Membranebound proteins MpgA and MpgB in Streptococcus pneumoniae are important in maintaining cell wall integrity. Both MpgA and MpgB are muramidases, cutting nascent peptidoglycan at different positions along the sugar backbone.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Multidisciplinary Sciences

Enterococcus peptidoglycan remodeling promotes checkpoint inhibitor cancer immunotherapy

Matthew E. Griffin et al.

Summary: This study found that members of the Enterococcus bacterial genus can improve checkpoint inhibitor immunotherapy in mouse tumor models through the expression and secretion of immune-active murpeptides. Additionally, expression of this enzyme in nonprotective bacteria can also enhance immunotherapy response, requiring another bacterial sensor for its activity.

SCIENCE (2021)

Article Microbiology

Discovery of a Diverse Set of Bacteria That Build Their Cell Walls without the Canonical Peptidoglycan Polymerase aPBP

Sharanjeet Atwal et al.

Summary: Peptidoglycan (PG) is a crucial component of bacterial cell walls, providing rigidity and shape. Some host-associated bacteria have lost genes for aPBPs while retaining bPBPs, and they build a minimal PG-like structure, potentially improving fitness by evading recognition by the host immune system.
Article Microbiology

Distinct Amino Acid Availability-Dependent Regulatory Mechanisms of MepS and MepM Levels in Escherichia coli

Yung Jae Kim et al.

Summary: Peptidoglycan hydrolases are crucial for bacterial physiology, with the two major endopeptidases, MepS and MepM, showing protein level regulation dependent on amino acid availability. The regulatory mechanisms and signaling pathways for MepS and MepM differ significantly, demonstrating the complexity of bacterial protein regulation mechanisms.

FRONTIERS IN MICROBIOLOGY (2021)

Article Chemistry, Multidisciplinary

Substrate and Stereochemical Control of Peptidoglycan Cross-Linking by Transpeptidation by Escherichia coli PBP1B

Anita C. Catherwood et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Multidisciplinary Sciences

Evolution-guided discovery of antibiotics that inhibit peptidoglycan remodelling

Elizabeth J. Culp et al.

NATURE (2020)

Article Multidisciplinary Sciences

Peptidoglycan reshaping by a noncanonical peptidase for helical cell shape in Campylobacter jejuni

Kyungjin Min et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

Remodeling of Cross-bridges Controls Peptidoglycan Cross-linking Levels in Bacterial Cell Walls

Alexis J. Apostolos et al.

ACS CHEMICAL BIOLOGY (2020)

Article Multidisciplinary Sciences

Structural basis of peptidoglycan endopeptidase regulation

Jung-Ho Shin et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Cell Biology

Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides

Sara B. Hernandez et al.

CELL REPORTS (2020)

Article Biochemistry & Molecular Biology

Facile Synthesis and Metabolic Incorporation of m-DAP B oisosteres Into Cell Walls of Live Bacteria

Alexis J. Apostolos et al.

ACS CHEMICAL BIOLOGY (2020)

Review Biochemistry & Molecular Biology

Bacterial Peptidoglycans from Microbiota in Neurodevelopment and Behavior

Ayoze Gonzalez-Santana et al.

TRENDS IN MOLECULAR MEDICINE (2020)

Review Microbiology

Bacterial Cell Wall Quality Control during Environmental Stress

Elizabeth A. Mueller et al.

Article Genetics & Heredity

MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA

Xiaolong Liu et al.

PLOS GENETICS (2020)

Review Microbiology

Peptidoglycan Muropeptides: Release, Perception, and Functions as Signaling Molecules

Oihane Irazoki et al.

FRONTIERS IN MICROBIOLOGY (2019)

Article Multidisciplinary Sciences

Peptidoglycan hydrolase of an unusual cross-link cleavage specificity contributes to bacterial cell wall synthesis

Pavan Kumar Chodisetti et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Review Microbiology

Bacteria's different ways to recycle their own cell wall

Christoph Mayer et al.

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY (2019)

Article Biochemistry & Molecular Biology

Lytic transglycosylases RlpA and MltC assist in Vibrio cholerae daughter cell separation

Anna Weaver et al.

MOLECULAR MICROBIOLOGY (2019)

Review Chemistry, Multidisciplinary

D-Amino Acid Derivatives as in Situ Probes for Visualizing Bacterial Peptidoglycan Biosynthesis

Yen-Pang Hsu et al.

ACCOUNTS OF CHEMICAL RESEARCH (2019)

Article Chemistry, Multidisciplinary

Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases

Michael A. Welsh et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

Article Biochemistry & Molecular Biology

Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis

Erkin Kuru et al.

ACS CHEMICAL BIOLOGY (2019)

Article Multidisciplinary Sciences

Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division

Martin Alcorlo et al.

NATURE COMMUNICATIONS (2019)

Article Biochemical Research Methods

False positives in using the zymogram assay for identification of peptidoglycan hydrolases

Cristian A. Escobar et al.

ANALYTICAL BIOCHEMISTRY (2018)

Article Biochemistry & Molecular Biology

Essentiality and function of WalK/WalR two-component system: the past, present, and future of research

Hiraku Takada et al.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2018)

Article Chemistry, Multidisciplinary

Synthesis of Functionalized N-Acetyl Muramic Acids To Probe Bacterial Cell Wall Recycling and Biosynthesis

Kristen E. DeMeester et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Multidisciplinary Sciences

Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa

Mijoon Lee et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Chemistry, Medicinal

Total Syntheses of Bulgecins A, B, and C and Their Bactericidal Potentiation of the β-Lactam Antibiotics

Shusuke Tomoshige et al.

ACS INFECTIOUS DISEASES (2018)

Review Biochemistry & Molecular Biology

Imaging Bacterial Cell Wall Biosynthesis

Atanas D. Radkov et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87 (2018)

Article Multidisciplinary Sciences

Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae

Cecile Morlot et al.

NATURE COMMUNICATIONS (2018)

Review Immunology

Peptidoglycan recognition by the innate immune system

Andrea J. Wolf et al.

NATURE REVIEWS IMMUNOLOGY (2018)

Article Biochemistry & Molecular Biology

Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B

Dustin T. King et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2017)

Article Chemistry, Multidisciplinary

Identification of a Functionally Unique Family of Penicillin-Binding Proteins

Michael A. Welsh et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Chemistry, Multidisciplinary

Peptidoglycan Cross-Linking Preferences of Staphylococcus aureus Penicillin-Binding Proteins Have Implications for Treating MRSA Infections

Veerasak Srisuknimit et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Biochemistry & Molecular Biology

Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams

Yuan Qiao et al.

NATURE CHEMICAL BIOLOGY (2017)

Article Multidisciplinary Sciences

Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis

Sophie Leclercq et al.

SCIENTIFIC REPORTS (2017)

Article Chemistry, Multidisciplinary

Synthesis and shift-reagent-assisted full NMR assignment of bacterial (Z8, E2, ω)-undecaprenol

Mijoon Lee et al.

CHEMICAL COMMUNICATIONS (2017)

Review Biochemistry & Molecular Biology

Lytic transglycosylases: concinnity in concision of the bacterial cell wall

David A. Dik et al.

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY (2017)

Article Chemistry, Multidisciplinary

From Genome to Proteome to Elucidation of Reactions for All Eleven Known Lytic Transglycosylases from Pseudomonas aeruginosa

Mijoon Lee et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Article Biochemistry & Molecular Biology

Identification of MltG as a potential terminase for peptidoglycan polymerization in bacteria

Rachel Yunck et al.

MOLECULAR MICROBIOLOGY (2016)

Article Biochemistry & Molecular Biology

A novel peptidoglycan D,L-endopeptidase induced by Salmonella inside eukaryotic cells contributes to virulence

Gadea Rico-Perez et al.

MOLECULAR MICROBIOLOGY (2016)

Article Biotechnology & Applied Microbiology

AcmB Is an S-Layer-Associated β-N-Acetylglucosaminidase and Functional Autolysin in Lactobacillus acidophilus NCFM

Brant R. Johnson et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2016)

Article Biochemistry & Molecular Biology

Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae

Jonathan D. Lenz et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

Article Infectious Diseases

Orthologous and Paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria

Ivanna Rivera et al.

MICROBIAL DRUG RESISTANCE (2016)

Article Multidisciplinary Sciences

SEDS proteins are a widespread family of bacterial cell wall polymerases

Alexander J. Meeske et al.

NATURE (2016)

Article Multidisciplinary Sciences

A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens

Kavita J. Rangan et al.

SCIENCE (2016)

Article Biotechnology & Applied Microbiology

Discovery of novel S-aureus autolysins and molecular engineering to enhance bacteriolytic activity

Daniel C. Osipovitch et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

A product of RpfB and RipA joint enzymatic action promotes the resuscitation of dormant mycobacteria

Vadim D. Nikitushkin et al.

FEBS JOURNAL (2015)

Article Biochemistry & Molecular Biology

The Cell Shape-determining Csd6 Protein from Helicobacter pylori Constitutes a New Family of L,D-Carboxypeptidase

Hyoun Sook Kim et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2015)

Article Biochemistry & Molecular Biology

Structural Analysis of a Specialized Type III Secretion System Peptidoglycan-cleaving Enzyme

Brianne J. Burkinshaw et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2015)

Article Multidisciplinary Sciences

Regulated proteolysis of a cross-link-specific peptidoglycan hydrolase contributes to bacterial morphogenesis

Santosh Kumar Singh et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Carbohydrate scaffolds as glycosyltransferase inhibitors with in vivo antibacterial activity

Johannes Zuegg et al.

NATURE COMMUNICATIONS (2015)

Article Microbiology

Fusion with a cell wall binding domain renders autolysin LytM a potent anti-Staphylococcus aureus agent

Daniel C. Osipovitch et al.

FEMS MICROBIOLOGY LETTERS (2015)

Article Biochemistry & Molecular Biology

Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli

Cecilia Artola-Recolons et al.

ACS CHEMICAL BIOLOGY (2014)

Article Biochemistry & Molecular Biology

The Essential Protein for Bacterial Flagella Formation FlgJ Functions as a β-N-Acetylglucosaminidase

Francesca A. Herlihey et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Structure-Function Analysis of Staphylococcus aureus Amidase Reveals the Determinants of Peptidoglycan Recognition and Cleavage

Felix Michael Buettner et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Article Chemistry, Multidisciplinary

Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis

Matthew D. Lebar et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Chemistry, Multidisciplinary

Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction

Yuan Qiao et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Biochemistry & Molecular Biology

Structure of the LdcB LD-Carboxypeptidase Reveals the Molecular Basis of Peptidoglycan Recognition

Christopher N. Hoyland et al.

STRUCTURE (2014)

Article Multidisciplinary Sciences

Molecular basis for bacterial peptidoglycan recognition by LysM domains

Stephane Mesnage et al.

NATURE COMMUNICATIONS (2014)

Article Biochemistry & Molecular Biology

In vitro Reconstitution of Peptidoglycan Assembly from the Gram-Positive Pathogen Streptococcus pneumoniae

Andre Zapun et al.

ACS CHEMICAL BIOLOGY (2013)

Article Biochemistry & Molecular Biology

Dual Substrate Specificity of Bacillus subtilis PBP4a

Venkatesh V. Nemmara et al.

BIOCHEMISTRY (2013)

Article Biochemistry & Molecular Biology

Secreted Proteases Control Autolysin-mediated Biofilm Growth of Staphylococcus aureus

Chen Chen et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2013)

Article Chemistry, Multidisciplinary

New Continuous Fluorometric Assay for Bacterial Transglycosylase Using Forster Resonance Energy Transfer

Shih-Hsien Huang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Tuning the Moenomycin Pharmacophore To Enable Discovery of Bacterial Cell Wall Synthesis Inhibitors

Christian M. Gampe et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Reactions of All Escherichia coli Lytic Transglycosylases with Bacterial Cell Wall

Mijoon Lee et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Reactions of the Three AmpD Enzymes of Pseudomonas aeruginosa

Weilie Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa

Siseth Martinez-Caballero et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa

Mijoon Lee et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Chemistry, Multidisciplinary

Forming Cross-Linked Peptidoglycan from Synthetic Gram-Negative Lipid II

Matthew D. Lebar et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Multidisciplinary Sciences

Kinetic Features of L,D-Transpeptidase Inactivation Critical for β-Lactam Antibacterial Activity

Sebastien Triboulet et al.

PLOS ONE (2013)

Article Chemistry, Multidisciplinary

Effect of the Peptide Moiety of Lipid II on Bacterial Transglycosylase

Hao-Wei Shih et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Chemistry, Multidisciplinary

In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D-Amino Acids

Erkin Kuru et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Biochemistry & Molecular Biology

Determinants of Murein Hydrolase Targeting to Cross-wall of Staphylococcus aureus Peptidoglycan

Matthew B. Frankel et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2012)

Article Biochemistry & Molecular Biology

Septal Localization of the Mycobacterium tuberculosis MtrB Sensor Kinase Promotes MtrA Regulon Expression

Renata Plocinska et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2012)

Article Chemistry, Multidisciplinary

The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment

Catherine Leimkuhler Grimes et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Article Biochemistry & Molecular Biology

Cooperativity of peptidoglycan synthases active in bacterial cell elongation

Manuel Banzhaf et al.

MOLECULAR MICROBIOLOGY (2012)

Article Biochemistry & Molecular Biology

Peptidoglycan maturation enzymes affect flagellar functionality in bacteria

Sophie Roure et al.

MOLECULAR MICROBIOLOGY (2012)

Article Biochemistry & Molecular Biology

Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12

Santosh Kumar Singh et al.

MOLECULAR MICROBIOLOGY (2012)

Article Biochemistry & Molecular Biology

A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division

Desiree C. Yang et al.

MOLECULAR MICROBIOLOGY (2012)

Article Multidisciplinary Sciences

Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism

Chia-Ying Huang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Biochemistry & Molecular Biology

Substrate Specificity of Low-Molecular Mass Bacterial DD-Peptidases

Venkatesh V. Nemmara et al.

BIOCHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids

Felipe Cava et al.

EMBO JOURNAL (2011)

Article Microbiology

AmpH, a Bifunctional DD-Endopeptidase and DD-Carboxypeptidase of Escherichia coli

Silvia M. Gonzalez-Leiza et al.

JOURNAL OF BACTERIOLOGY (2011)

Article Biochemistry & Molecular Biology

Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an Unprecedented Activation Mechanism

Cesar Carrasco-Lopez et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

LytN, a Murein Hydrolase in the Cross-wall Compartment of Staphylococcus aureus, Is Involved in Proper Bacterial Growth and Envelope Assembly

Matthew B. Frankel et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Peptidoglycan Remodeling in Mycobacterium tuberculosis: Comparison of Structures and Catalytic Activities of RipA and RipB

Dominic Both et al.

JOURNAL OF MOLECULAR BIOLOGY (2011)

Article Chemistry, Multidisciplinary

Primer Preactivation of Peptidoglycan Polymerases

Tsung-Shing Andrew Wang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

Article Chemistry, Multidisciplinary

Transpeptidase-Mediated Incorporation of D-Amino Acids into Bacterial Peptidoglycan

Tania J. Lupoli et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

Article Multidisciplinary Sciences

An ATP-binding cassette transporter-like complex governs cell-wall hydrolysis at the bacterial cytokinetic ring

Desiree C. Yang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Biochemistry & Molecular Biology

Bacillus subtilis Cw1Q (previous YjbJ) is a bifunctional enzyme exhibiting muramidase and soluble-lytic transglycosylase activities

I. Putu Sudiarta et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2010)

Article Biochemistry & Molecular Biology

Daughter cell separation is controlled by cytokinetic ring-activated cell wall hydrolysis

Tsuyoshi Uehara et al.

EMBO JOURNAL (2010)

Article Chemistry, Multidisciplinary

The Role of the Substrate Lipid in Processive Glycan Polymerization by the Peptidoglycan Glycosyltransferases

Deborah L. Perlstein et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

Article Biochemistry & Molecular Biology

A M23B family metallopeptidase of Helicobacter pylori required for cell shape, pole formation and virulence

Mathilde Bonis et al.

MOLECULAR MICROBIOLOGY (2010)

Article Microbiology

Structural Basis of Cell Wall Cleavage by a Staphylococcal Autolysin

Sebastian Zoll et al.

PLOS PATHOGENS (2010)

Article Biochemistry & Molecular Biology

Development of a novel fluorescent substrate for Autolysin E, a bacterial type II amidase

Nicolas Luetzner et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2009)

Article Microbiology

Roles of Germination-Specific Lytic Enzymes CwlJ and SleB in Bacillus anthracis

Jared D. Heffron et al.

JOURNAL OF BACTERIOLOGY (2009)

Article Chemistry, Multidisciplinary

Bacterial AmpD at the Crossroads of Peptidoglycan Recycling and Manifestation of Antibiotic Resistance

Mijoon Lee et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Chemistry, Multidisciplinary

Studying a Cell Division Amidase Using Defined Peptidoglycan Substrates

Tania J. Lupoli et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Multidisciplinary Sciences

Crystal structure of the membrane-bound bifunctional transglycosylase PBP1b from Escherichia coli

Ming-Ta Sung et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Review Biochemistry & Molecular Biology

Murein (peptidoglycan) structure, architecture and biosynthesis in Escherichia coli

Waldemar Vollmer et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2008)

Article Microbiology

Neisseria gonorrhoeae uses two lytic transglycosylases to produce cytotoxic peptidoglycan monomers

Karen A. Cloud-Hansen et al.

JOURNAL OF BACTERIOLOGY (2008)

Article Biochemistry & Molecular Biology

Role of N-acetylglucosaminidase and N-acetylmuramidase activities in Enterococcus faecalis peptidoglycan metabolism

Stephane Mesnage et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2008)

Article Biochemistry & Molecular Biology

Identification of Dynamic Structural Motifs Involved in Peptidoglycan Glycosyltransfer

Andrew L. Lovering et al.

JOURNAL OF MOLECULAR BIOLOGY (2008)

Article Chemistry, Multidisciplinary

Isolated Peptidoglycan Glycosyltransferases from Different Organisms Produce Different Glycan Chain Lengths

Tsung-Shing Andrew Wang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)

Review Microbiology

Peptidoglycan structure and architecture

Waldemar Vollmer et al.

FEMS MICROBIOLOGY REVIEWS (2008)

Review Microbiology

Structural variation in the glycan strands of bacterial peptidoglycan

Waldemar Vollmer

FEMS MICROBIOLOGY REVIEWS (2008)

Article Biochemistry & Molecular Biology

Analysis of glycan polymers produced by peptidoglycan glycosyltransferases

Dianah Barrett et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2007)

Article Chemistry, Multidisciplinary

The direction of glycan chain elongation by peptidoglycan Glycosyltransferases

Deborah L. Perlstein et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)

Article Biochemistry & Molecular Biology

Unexpected inhibition of peptidoglycan LD-transpeptidase from enterococcus faecium by the β-lactam imipenem

Jean-Luc Mainardi et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2007)

Article Microbiology

Characterization of IsaA and SceD, two putative lytic transglycosylases of Staphylococcus aureus

Melanie R. Stapleton et al.

JOURNAL OF BACTERIOLOGY (2007)

Article Biochemistry & Molecular Biology

Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis

Tatsuya Fukushima et al.

MOLECULAR GENETICS AND GENOMICS (2007)

Article Biochemistry & Molecular Biology

Reactions of peptidoglycan-mimetic β-lactams with penicillin-binding proteins in vivo and in membranes

Ish Kumar et al.

ACS CHEMICAL BIOLOGY (2007)

Article Biochemistry & Molecular Biology

The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis

Paola Bisicchia et al.

MOLECULAR MICROBIOLOGY (2007)

Article Biochemistry & Molecular Biology

Specificity of L,D-transpeptidases from gram-positive bacteria producing different peptidoglycan chemotypes

Sophie Magnet et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2007)

Article Multidisciplinary Sciences

Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis

Yanqiu Yuan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Chemistry, Multidisciplinary

Synthesis of heptaprenyl-lipid IV to analyze peptidoglycan glycosyltransferases

Yi Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)

Article Multidisciplinary Sciences

Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis

Andrew L. Lovering et al.

SCIENCE (2007)

Article Biochemistry & Molecular Biology

The Enterococcus hirae Mur-2 enzyme displays N-acetylglucosaminidase activity

Catherine Eckert et al.

FEBS LETTERS (2007)

Article Microbiology

Functional analysis of AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis

Catherine Eckert et al.

JOURNAL OF BACTERIOLOGY (2006)

Article Biochemistry & Molecular Biology

In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli

Petra Born et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2006)

Article Microbiology

Activity of the major staphylococcal autolysin Atl

Raja Biswas et al.

FEMS MICROBIOLOGY LETTERS (2006)

Article Biochemistry & Molecular Biology

A novel peptidoglycan cross-linking enzyme for a β-lactam-resistant transpeptidation pathway

JL Mainardi et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2005)

Article Biochemistry & Molecular Biology

Synthesis of mosaic peptidoglycan cross-bridges by hybrid peptidoglycan assembly pathways in gram-positive bacteria

A Arbeloa et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2004)

Article Chemistry, Multidisciplinary

The perfect penicillin?: Inhibition of a bacterial DD-peptidase by peptidoglycan-mimetic β-lactams

HR Josephine et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)

Article Biotechnology & Applied Microbiology

Analysis of the peptidoglycan hydrolase complement of Lactococcus lactis:: Identification of a third N-acetylglucosaminidase, AcmC

C Huard et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2004)

Article Chemistry, Multidisciplinary

A mechanism-based inhibitor targeting the DD-transpeptidase activity of bacterial penicillin-binding proteins

M Lee et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)

Article Biochemistry & Molecular Biology

Synthesis of the L-alanyl-L-alanine cross-bridge of Enterococcus faecalis peptidoglycan

A Bouhss et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2002)

Article Biochemistry & Molecular Biology

Balance between two transpeptidation mechanisms determines the expression of β-lactam resistance in Enterococcus faecium

JL Mainard et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2002)

Article Chemistry, Multidisciplinary

Better substrates for bacterial transglycosylases

XY Ye et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2001)

Article Biochemistry & Molecular Biology

Novel mechanism of β-lactam resistance due to bypass of DD-transpeptidation in Enterococcus faecium

JL Mainardi et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2000)