Journal
CARBOHYDRATE POLYMERS
Volume 297, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.120049
Keywords
Chitosan; Cell adhesion factors; Neuronal cultures; Neurite outgrowth; Axonal polarity; Electrophysiological behavior
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In this study, the use of chitosan as an adhesion factor for neuronal growth and differentiation was investigated. The results showed that chitosan promoted neuritic processes formation and neuronal polarity, suggesting its potential as an alternative to standard adhesion factors. Additionally, chitosan can be used in both 2D and 3D cultures, bridging the gap between different in vitro models.
Most in vitro functional and morphological studies for developing nervous system have been performed using traditional monolayer cultures onto supports modified by extracellular matrix components or synthetic bio-polymers. These biomolecules act as adhesion factors essential for neuronal growth and differentiation. In this study, the use of chitosan as adhesion factor was investigated. Primary rat neurons and neurons differentiated from human induced pluripotent stem cells were cultured onto chitosan and standard adhesion factors modified supports. The initiation, elongation and branching of neuritic processes, synaptogenesis and electrophysiological behavior were studied. The biopolymers affected neurites outgrowth in a time dependent manner; in particular, chitosan promoted neuronal polarity in both cell cultures. These results indicate chitosan as a valid adhesion factor alternative to the standard ones, with the advantage that it can be used both in 2D and 3D cultures, acting as a bridge between these in vitro models.
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