Journal
CARBOHYDRATE POLYMERS
Volume 301, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.120318
Keywords
Burkholderia cenocepacia; Biofilm; bepA-L gene cluster; Exopolysaccharide structure; NMR spectroscopy
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Burkholderia cenocepacia is an opportunistic pathogen that causes in-fections in cystic fibrosis patients. Biofilm and its associated exopolysaccharides play a crucial role in infection persistence and antibiotic tolerance. This study identified the water-insoluble exopolysaccharide encoded by the B. cenocepacia H111 bepA-L gene cluster and highlighted its potential importance in in vivo infections.
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes in-fections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibi-otics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
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