Polyethylene terephthalate hydrolysate increased bacterial cellulose production

A major challenge to large-scale production and utilization of bacterial cellulose (BC) for various applications is its low yield and productivity by bacterial cells and the high cost of feedstock. A supplementation of the classical expensive Hestrin and Schramm (HS) medium with 1 % polyethylene terephthalate ammonia hydrolysate (PETAH) resulted in 215 % high yield. Although the physicochemical properties of BC were not significantly influenced, the BC produced in 1 % PETAH-supplemented HS medium showed a higher surface area, which showed 1.39 times higher adsorption capacity for tetracycline than BC produced in HS medium. The 1 % PETAHsupplemented HS medium respectively enhanced the activity of & alpha;-UDP-glucose pyrophosphorylase and & alpha;-phosphoglucomutase by 30.63 % and 135.24 % and decreased the activity of pyruvate kinase and phosphofructokinase by 40.34 % and 52.63 %. The results of this study provide insights into the activation mechanism of Taonella mepensis by PETAH supplementation for high yield and productivity of BC.

Automated summary

A major challenge in the large-scale production and utilization of bacterial cellulose (BC) is the low yield and productivity of bacterial cells and the high cost of feedstock. However, supplementation of the expensive Hestrin and Schramm (HS) medium with 1% polyethylene terephthalate ammonia hydrolysate (PETAH) led to a 215% increase in BC yield. The BC produced in the 1% PETAH-supplemented HS medium showed a higher surface area and a 1.39 times higher adsorption capacity for tetracycline compared to BC produced in the HS medium. Additionally, the 1% PETAH-supplemented HS medium enhanced the activity of key enzymes involved in BC production but decreased the activity of other enzymes. These findings provide insights into the activation mechanism of PETAH supplementation for high BC yield and productivity in Taonella mepensis.