4.2 Article

The Role of NO Synthase in the Infarct-Limiting Effect of Urgent and Chronic Adaptation to Normobaric Hypoxia

Journal

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 174, Issue 3, Pages 304-307

Publisher

SPRINGER
DOI: 10.1007/s10517-023-05696-3

Keywords

heart; ischemia; reperfusion; hypoxic adaptation; NO synthase

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The role of NO synthase in the infarct-limiting effect of short-term (SNH) and chronic continuous normobaric hypoxia (CNH) was studied. It was found that non-selective NO synthase inhibitor L-NAME and inducible NO synthase inhibitor S-methylthiourea abolished the infarct-limiting effect of SNH and CNH. Nitric oxide donor diethylenetriamine increased cardiac tolerance to ischemia/reperfusion. Inducible NO synthase is believed to play an important role in the cardioprotective effect of normobaric hypoxia.
We studied the role of NO synthase in the infarct-limiting effect of short-term (SNH) and chronic continuous normobaric hypoxia (CNH). In male Wistar rats, SNH (6 sessions of 10-min hypoxia 8% O2 and 10-min reoxygenation) or CNH (12% O2 for 21 days) was modeled. In 30 min after SNH or 24 h after CNH, the rats were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). The following drugs were administered to rats: non-selective NO synthase inhibitor L-NAME (10 mg/kg), inhibitor of inducible NO synthase S-methylthiourea (3 mg/kg), and inhibitor of neuronal NO-synthase 7-nitroindazole (50 mg/kg). NO donor diethylenetriamine was administered intravenously in a dose 2 mg/kg. It was found that L-NAME and S-methylthiourea abolished the infarct-limiting effect of SNH and CNH. Diethylenetriamine increased cardiac tolerance to ischemia/reperfusion. It is believed that inducible NO synthase plays an important role in the cardioprotective effect of normobaric hypoxia.

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