Journal
BMC GENOMICS
Volume 23, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12864-022-08966-y
Keywords
Genome; Biocontrol; Olive knot disease; Antibiotics biosynthesis; Integrative conjugative element; ICE; Secretion systems
Funding
- Department of Life Sciences and Facility Management of the Zurich University of Applied Sciences (ZHAW) in Wadenswil, Switzerland
- BMBF within the de.NBI network [FKZ031A533]
- Department of Agricultural, Food and Environmental Sciences (DSA3) of the University of Perugia (Italy)
Ask authors/readers for more resources
In this study, the whole genome of Pantoea agglomerans DAPP-PG 734, which was isolated from knots in olive trees caused by Pseudomonas savastanoi pv. savastanoi DAPP-PG 722, was sequenced and analyzed. Gene clusters related to enhanced interaction and antibiotic synthesis were identified. The in-depth analysis of the genome suggested that Pantoea agglomerans DAPP-PG 734 is an endophytic bacterium with biocontrol activity.
Pantoea agglomerans DAPP-PG 734 was isolated as endophyte from knots (tumors) caused by Pseudomonas savastanoi pv. savastanoi DAPP-PG 722 in olive trees. To understand the plant pathogen-endophyte interaction on a genomic level, the whole genome of P. agglomerans DAPP-PG 734 was sequenced and annotated. The complete genome had a total size of 5 ' 396 ' 424 bp, containing one circular chromosome and four large circular plasmids. The aim of this study was to identify genomic features that could play a potential role in the interaction between P. agglomerans DAPP-PG 734 and P. savastanoi pv. savastanoi DAPP-PG 722. For this purpose, a comparative genomic analysis between the genome of P. agglomerans DAPP-PG 734 and those of related Pantoea spp. was carried out. In P. agglomerans DAPP-PG 734, gene clusters for the synthesis of the Hrp-1 type III secretion system (T3SS), type VI secretion systems (T6SS) and autoinducer, which could play an important role in a plant-pathogenic community enhancing knot formation in olive trees, were identified. Additional gene clusters for the biosynthesis of two different antibiotics, namely dapdiamide E and antibiotic B025670, which were found in regions between integrative conjugative elements (ICE), were observed. The in-depth analysis of the whole genome suggested a characterization of the P. agglomerans DAPP-PG 734 isolate as endophytic bacterium with biocontrol activity rather than as a plant pathogen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available