Advances in enzyme biocatalysis for the preparation of functional lipids

Functional lipids, mainly omega-3 polyunsaturated fatty acids (n-3 PUFAs) such as eicosapentaenoic (EPA; 20:5n-3) and docosahexaenoic (DHA; 22:6n-3), are known to have a variety of health benefits. Lipases and phospholipases are widely used to prepare different forms of structured lipids, since biocatalytic methods can be carried out under mild conditions, preserving the quality of the products. On the other hand, many processes still are conducted at high temperatures and with organic solvents, which are conditions unfavorable for the production of nutritional products. This article gives an updated overview of enzyme biocatalysis methods for the prepa-ration of different derivatives containing n-3 PUFAs, including specific reactions, enzyme immobilization research for high-efficiency catalysis, and enzyme engineering technologies (higher selectivity, stability, and activity). Furthermore, advanced control strategies of biocatalytic processes and reactors are presented. The future prospect and opportunities for marine functional lipids are also discussed. Therefore, the obtainment of enzymes endowed with superior properties and the development of optimized processes, still have to be pursued to achieve greener bio-catalyzed processes.

Automated summary

This article provides an updated overview of enzyme biocatalysis methods for the preparation of derivatives containing n-3 polyunsaturated fatty acids. It discusses specific reactions, enzyme immobilization research, and enzyme engineering technologies. The article also presents advanced control strategies for biocatalytic processes and reactors, and explores the future prospects and opportunities for marine functional lipids.