Single-neuron detection of place cells remapping in short-term memory using motion microelectrode arrays

Place cells establish rapid mapping relationships between the external environment and themselves in a new context. However, the mapping relationships of environmental cues to place cells in short-term memory is still completely unknown. In this work, we designed a silicon-based motion microelectrode array (mMEA) and an implantation device to record electrophysiological signals of place cells in CA1, CA3, and DG regions in the hippocampus of ten mice in motion, and investigated the corresponding place fields under distal or local cues in just a few minutes. The mMEA can expand the detection area and greatly lower the motion noise. Finding and recording place cells of moving mice in short-term memory is made possible by the mMEA. The place-related cells were found for the first time. Unlike place cells, which only fire in a particular position of the environment, place-related cells fire in numerous areas of the environment. Furthermore, place cells in the CA1 and CA3 have the most stable place memory for time-preferred single cues, and they fire in concert with place-related cells during short-term memory dynamics, whereas place cells in the DG regions have overlapping and unstable place memory in a multi-cue context. These results demonstrate the consistency of place cells in CA1 and CA3 and reflect their different roles in spatial memory processing during familiarization with new environments. The mMEA provides a platform for studying the place cells of short-term memory.

Automated summary

This study utilized a silicon-based motion microelectrode array to record electrophysiological signals of place cells in the hippocampus of mice in motion. The results revealed the mapping relationships between environmental cues and place cells in short-term memory and identified the existence of place-related cells. Furthermore, the study demonstrated the different roles of place cells in CA1, CA3, and DG regions in spatial memory processing during familiarization with new environments.