Journal
BIORESOURCE TECHNOLOGY
Volume 365, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.128172
Keywords
2-PDO; Lactate; Methane; Methanol; Glycerol; Propane; Type I methanotrophs
Funding
- C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2015M3D3A1A01064882]
- Korea Institute of Marine Science and Technology Promotion (KIMST) - Ministry of Oceans and Fisheries, Korea [20220532]
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The study explores the metabolic pathways of methane and methanol to produce 1,2-propanediol (1,2-PDO) using the type I methanotroph Methylotuvimicrobium alcaliphilum 20Z. It finds that the carbon flux from methane to the EMP pathway restricts the production of 1,2-PDO. Co-utilization of C1 substrates and glycerol enhances the production of 1,2-PDO, although lactate is co-produced in higher amounts. The study provides insights into metabolic routes for 1,2-PDO and lactate co-production from C1 and C3 compounds in methanotrophs.
Methanotrophic bacteria are attractive hosts for mining metabolic pathways of C1 assimilation to produce valueadded products. Herein, the type I methanotroph Methylotuvimicrobium alcaliphilum 20Z was employed to explore the carbon flux from methane and methanol via the EMP pathway to produce 1,2-propanediol (1,2-PDO). The production of 1,2-PDO on methane was found to be mainly restricted by the lower carbon flux toward the EMP pathway. The co-utilization of C1 substrates and glycerol (C3) could contribute to enhance 1,2-PDO. Lactate was co-produced in much higher amounts than 1,2-PDO. This unexpected product was probably derived from lactaldehyde by inherent aldehyde dehydrogenases. The 1,2-PDO production without increased accumulation of lactate was observed via establishing the acetol-based pathway by propane utilization with the overexpression of pmoD. This is the first study to provide experimental insights into the operation of metabolic routes for 1,2-PDO and lactate co-production from C1 and C3 compounds in methanotrophs.
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